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  • 产品名称：Alpha-(1,3)-Fucosyltransferase 4 (FUT4) Antibody

  • 产品型号：abx227136
  • 产品厂商：abbexa
  • 产品文档：
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简单介绍：
Alpha-(1,3)-Fucosyltransferase 4 (FUT4) Antibody
详情介绍：
Alpha-(1,3)-Fucosyltransferase 4 (FUT4) Antibody is a Rabbit Monoclonal antibody for the detection of CD15.

Target Alpha-(1,3)-Fucosyltransferase 4 (FUT4)
Clonality Monoclonal
Clone W081
Reactivity Human
Tested Applications IHC
Host Rabbit
Recommended dilutions IHC-P: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Synthetic peptide derived from the N-terminal region of human CD15.
Isotype IgG
Form Liquid
Purification Purified from rabbit antiserum by proprietary techniques.
Storage Store at 2-8°C.
UniProt Primary AC P22083 (UniProt, ExPASy)
UniProt Secondary AC B2RMS0
UniProt Entry Name FUT4_HUMAN
Gene Symbol FUT4
GeneID 2526
OMIM 104230
HGNC 4015
KEGG hsa:2526
Ensembl ENSG00000196371
String 9606.ENSP00000351602
Buffer 20 mM Tris-HCl, pH 8.0, containing 20 mg/ml BSA and 0.05% NaN3.
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Directions for use Suggested IHC-P Protocol
  • Preparation of Citrate Buffer (10 mM Citric Acid, 0.05% Tween-20, pH 6.0): Dissolve 1.92 g of anhydrous citric acid in 700 ml of distilled water. Adjust pH to 6.0 with 1 M NaOH and then add 0.5 ml of Tween-20 and mix thoroughly. Adjust the final volume to 1 L with distilled water. Store this solution at room temperature for up to 3 months or at 4 °C for long-term storage.
  • Preparation of Tris-EDTA Buffer (10 mM Tris Base, 1 mM EDTA solution, 0.05% Tween-20, pH 9.0): Mix 1.21 g Tris and 0.37 g EDTA and dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1 M HCl and then add 0.5 ml of Tween-20 and mix thoroughly. Adjust the final volume to 1 L with distilled water. Store this solution at room temperature for up to 3 months or at 4 °C for long-term storage.
  • Preparation of Wash Buffer: Use PBS, pH 7.0-7.5, containing 0.05% Tween-20.
  • Deparaffinize the section in 3 changes of xylene, 10 minutes each.
  • Wash the section in 96%, 80% and 70% ethanol, 10 minutes each.
  • Rinse twice in distilled water, 5 minutes each.
  • Block endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
  • Wash twice in distilled water, 5 minutes each.
  • Antigen retrieval: immerse the slide in either Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 9.0), and incubate in a water bath for 20-30 minutes at 96-98 °C.
  • Remove the slide from the water bath and allow to stand at room temperature for 15 minutes in the buffer used in the previous step.
  • Rinse twice in distilled water, 5 minutes each.
  • Wash twice in Wash Buffer, 5 minutes each.
  • Incubate the section with primary antibody at 1/100 - 1/200 dilution for 1 hour in a closed wet chamber. It is recommended to use abx291502 Primary Antibody Diluent or a diluent containing protease-free BSA (> 1 mg/ml) to dilute this antibody.
  • Wash 3 times with Wash Buffer, 5 minutes each.
  • Add the secondary antibody and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). It is recommended to use abx291501 Rabbit and Mouse HRP/DAB Detection Kit.
  • Wash 3 times in Wash Buffer, 5 minutes each.
  • Apply the DAB chromagen for 1-3 minutes.
  • Wash twice in distilled water, 5 minutes each.
  • Stain in hematoxylin for 5 minutes.
  • Wash 3 times in distilled water, 2 minutes each.
  • Mount the slide for observation.
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