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  • 产品名称：Human ABCA1 (Phospholipid-transporting ATPase ABCA1) Sandwich ELISA (STJE0002297)

  • 产品型号：STJE0002297
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human ABCA1 (Phospholipid-transporting ATPase ABCA1) Sandwich ELISA (STJE0002297)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This ABCA1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: ABCA1
Gene ID: 19
Uniprot ID: ABCA1_HUMAN
Specificity: This kit recognizes Human ABCA1 in samples. No significant cross-reactivity or interference between Human ABCA1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylation on Ser-2054 regulates phospholipid efflux. Palmitoylated by ZDHHC8. Palmitoylation is essential for localization to the plasma membrane.
Function Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP. Thereby, participates in phospholipid transfer to apolipoproteins to form nascent high density lipoproteins/HDLs. Transports preferentially phosphatidylcholine over phosphatidylserine. May play a similar role in the efflux of intracellular cholesterol to apolipoproteins and the formation of nascent high density lipoproteins/HDLs. Translocates phospholipids from the outer face of the plasma membrane and forces it through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain.
Protein Name Phospholipid-Transporting Atpase Abca1
Atp-Binding Cassette Sub-Family A Member 1
Atp-Binding Cassette Transporter 1
Abc-1
Atp-Binding Cassette 1
Cholesterol Efflux Regulatory Protein
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-5682113
Reactome: R-HSA-8963896
Reactome: R-HSA-9029569
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Endosome
Alternative ELISA Names Phospholipid-Transporting Atpase Abca1 ELISA kit
Atp-Binding Cassette Sub-Family A Member 1 ELISA kit
Atp-Binding Cassette Transporter 1 ELISA kit
Abc-1 ELISA kit
Atp-Binding Cassette 1 ELISA kit
Cholesterol Efflux Regulatory Protein ELISA kit
ABCA1 ELISA kit
ABC1 ELISA kit
CERP ELISA kit
Specificity This kit recognizes Human ABCA1 in samples. No significant cross-reactivity or interference between Human ABCA1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-105 97
EDTA plasma (n=8) 97-109 102
Cell culture media (n=8) 94-110 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.47 1.35 3.56 0.51 1.23 3.38
Standard deviation 0.02 0.06 0.11 0.03 0.07 0.14
CV (%) 4.26 4.44 3.09 5.88 5.69 4.14
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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