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  • 产品名称：Human ACO1 (Cytoplasmic aconitate hydratase) CLIA Kit (STJC0000213)

  • 产品型号：STJC0000213
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human ACO1 (Cytoplasmic aconitate hydratase) CLIA Kit (STJC0000213)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human ACO1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: ACO1
Gene ID: 48
Uniprot ID: ACOHC_HUMAN
Specificity: This kit recognizes Human ACO1 in samples. No significant cross-reactivity or interference between Human ACO1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA. Conversely, when cellular iron levels are high, binds a 4Fe-4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis-aconitate.
Protein Name Cytoplasmic Aconitate Hydratase
Aconitase
Citrate Hydro-Lyase
Ferritin Repressor Protein
Iron Regulatory Protein 1
Irp1
Iron-Responsive Element-Binding Protein 1
Ire-Bp 1
Database Links Reactome: R-HSA-917937
Cellular Localisation Cytoplasm
Cytosol
Alternative CLIA Names Cytoplasmic Aconitate Hydratase CLIA kit
Aconitase CLIA kit
Citrate Hydro-Lyase CLIA kit
Ferritin Repressor Protein CLIA kit
Iron Regulatory Protein 1 CLIA kit
Irp1 CLIA kit
Iron-Responsive Element-Binding Protein 1 CLIA kit
Ire-Bp 1 CLIA kit
ACO1 CLIA kit
IREB1 CLIA kit
Specificity This kit recognizes Human ACO1 in samples. No significant cross-reactivity or interference between Human ACO1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-106 100
EDTA plasma(n=8) 98-110 104
Cell culture media(n=8) 92-105 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 106.87 286.48 886.35 115.85 276.99 826.38
Standard deviation 11.42 28.39 70.64 12.91 25.46 50.24
CV (%) 10.69 9.91 7.97 11.14 9.19 6.08
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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