产品详情
查看大图

  • 产品名称：Human ADRb2 (Adrenergic Receptor Beta 2) Sandwich ELISA Kit (STJE0006962)

  • 产品型号：STJE0006962
  • 产品厂商：ST john's laboratory
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
Human ADRb2 (Adrenergic Receptor Beta 2) Sandwich ELISA Kit (STJE0006962)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.114ng/mL
Detection Limit: 0.312-20ng/mL
Short Description: This ADRb2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: ADRB2
Gene ID: 154
Uniprot ID: ADRB2_HUMAN
Sample Type: serum, plasma, tissue homogenates or other biological fluids.
Tissue Specificity
Post Translational Modifications Palmitoylated. Mainly palmitoylated at Cys-341. Palmitoylation may reduce accessibility of phosphorylation sites by anchoring the receptor to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation. Also undergoes transient, ligand-induced palmitoylation at Cys-265 probably by ZDHHC9, ZDHHC14 and ZDHHC18 within the Golgi. Palmitoylation at Cys-265 requires phosphorylation by PKA and receptor internalization and stabilizes the receptor. Could be depalmitoylated by LYPLA1 at the plasma membrane. Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor. Polyubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation. Ubiquitination by the VHL-E3 ligase complex is oxygen-dependent. Hydroxylation by EGLN3 occurs only under normoxia and increases the interaction with VHL and the subsequent ubiquitination and degradation of ADRB2.
Function Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
Protein Name Beta-2 Adrenergic Receptor
Beta-2 Adrenoreceptor
Beta-2 Adrenoceptor
Database Links Reactome: R-HSA-390696
Reactome: R-HSA-418555
Reactome: R-HSA-5689880
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Early Endosome
Golgi Apparatus
Colocalizes With Vhl At The Cell Membrane
Activated Receptors Are Internalized Into Endosomes Prior To Their Degradation In Lysosomes
Activated Receptors Are Also Detected Within The Golgi Apparatus
Alternative ELISA Names Beta-2 Adrenergic Receptor ELISA kit
Beta-2 Adrenoreceptor ELISA kit
Beta-2 Adrenoceptor ELISA kit
ADRB2 ELISA kit
ADRB2R ELISA kit
B2AR ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
姓名：
电话：
您的需求：
 

沪公网安备 31011002002624号