产品详情
简单介绍：
Human ALPL (Alkaline phosphatase, tissue-nonspecific isozyme) Sandwich ELISA (STJE0003794)
详情介绍：
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 46.88pg/mL |
| Detection Limit: | 78.13~5000pg/mL |
| Short Description: | This ALPL Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | ALPL |
| Gene ID: | 249 |
| Uniprot ID: | PPBT_HUMAN |
| Specificity: | This kit recognizes Human ALPL in samples. No significant cross-reactivity or interference between Human ALPL and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Alkaline phosphatase that metabolizes various phosphate compounds and plays a key role in skeletal mineralization and adaptive thermogenesis. Has broad substrate specificity and can hydrolyze a considerable variety of compounds: however, only a few substrates, such as diphosphate (inorganic pyrophosphate.PPi), pyridoxal 5'-phosphate (PLP) and N-phosphocreatine are natural substrates. Plays an essential role in skeletal and dental mineralization via its ability to hydrolyze extracellular diphosphate, a potent mineralization inhibitor, to phosphate: it thereby promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration. Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix. Also promotes dephosphorylation of osteopontin (SSP1), an inhibitor of hydroxyapatite crystallization in its phosphorylated state.it is however unclear whether ALPL/TNAP mediates SSP1 dephosphorylation via a direct or indirect manner. Catalyzes dephosphorylation of PLP to pyridoxal (PL), the transportable form of vitamin B6, in order to provide a sufficient amount of PLP in the brain, an essential cofactor for enzymes catalyzing the synthesis of diverse neurotransmitters. Additionally, also able to mediate ATP degradation in a stepwise manner to adenosine, thereby regulating the availability of ligands for purinergic receptors. Also capable of dephosphorylating microbial products, such as lipopolysaccharides (LPS) as well as other phosphorylated small-molecules, such as poly-inosine:cytosine (poly I:C). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating hydrolysis of N-phosphocreatine to initiate a futile cycle of creatine dephosphorylation and phosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work. |
| Protein Name | Alkaline Phosphatase - Tissue-Nonspecific Isozyme Ap-Tnap Tns-Alp Tnsalp Alkaline Phosphatase Liver/Bone/Kidney Isozyme Phosphoamidase Phosphocreatine Phosphatase |
| Database Links | Reactome: R-HSA-163125 |
| Cellular Localisation | Cell Membrane Lipid-Anchor Gpi-Anchor Extracellular Vesicle Membrane Mitochondrion Membrane Mitochondrion Intermembrane Space Localizes To Special Class Of Extracellular Vesicles Named Matrix Vesicles (Mvs) Which Are Released By Osteogenic Cells Localizes To The Mitochondria Of Thermogenic Fat Cells: Tethered To Mitochondrial Membranes Via A Gpi-Anchor And Probably Resides In The Mitochondrion Intermembrane Space |
| Alternative ELISA Names | Alkaline Phosphatase - Tissue-Nonspecific Isozyme ELISA kit Ap-Tnap ELISA kit Tns-Alp ELISA kit Tnsalp ELISA kit Alkaline Phosphatase Liver/Bone/Kidney Isozyme ELISA kit Phosphoamidase ELISA kit Phosphocreatine Phosphatase ELISA kit ALPL ELISA kit |
| Specificity | This kit recognizes Human ALPL in samples. No significant cross-reactivity or interference between Human ALPL and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 96-110 | 102 |
| EDTA plasma (n=8) | 87-103 | 94 |
| Cell culture media (n=8) | 94-105 | 100 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 232.41 | 686.93 | 2152.29 | 235.06 | 692.44 | 2121.00 |
| Standard deviation | 15.27 | 28.64 | 98.79 | 13.26 | 38.85 | 75.51 |
| CV (%) | 6.57 | 4.17 | 4.59 | 5.64 | 5.61 | 3.56 |
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