产品详情
简单介绍:
Human APOA2 (Apolipoprotein A-II) Sandwich ELISA (STJE0004051)
详情介绍:
Applications: | ELISA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 18.75ng/mL |
Detection Limit: | 31.25~2000ng/mL |
Short Description: | This APOA2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | APOA2 |
Gene ID: | 336 |
Uniprot ID: | APOA2_HUMAN |
Specificity: | This kit recognizes Human ApoA2 in samples. No significant cross-reactivity or interference between Human ApoA2 and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Post Translational Modifications | Phosphorylation sites are present in the extracellular medium. Apolipoprotein A-II is O-glycosylated. |
Function | May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. |
Protein Name | Apolipoprotein A-Ii Apo-Aii Apoa-Ii Apolipoprotein A2 Cleaved Into - Proapolipoprotein A-Ii Proapoa-Ii - Truncated Apolipoprotein A-Ii Apolipoprotein A-Ii(1-76 |
Database Links | Reactome: R-HSA-1989781 Reactome: R-HSA-381426 Reactome: R-HSA-8957275 Reactome: R-HSA-8963888 Reactome: R-HSA-8963901 Reactome: R-HSA-975634 |
Cellular Localisation | Secreted |
Alternative ELISA Names | Apolipoprotein A-Ii ELISA kit Apo-Aii ELISA kit Apoa-Ii ELISA kit Apolipoprotein A2 Cleaved Into - Proapolipoprotein A-Ii ELISA kit Proapoa-Ii - Truncated Apolipoprotein A-Ii ELISA kit Apolipoprotein A-Ii(1-76 ELISA kit APOA2 ELISA kit |
Specificity | This kit recognizes Human ApoA2 in samples. No significant cross-reactivity or interference between Human ApoA2 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Serum(n=8) | 89-99 | 94 |
EDTA plasma (n=8) | 96-109 | 101 |
Cell culture media (n=8) | 93-103 | 98 |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 96.14 | 224.95 | 844.46 | 86.28 | 245.63 | 844.50 |
Standard deviation | 5.52 | 10.12 | 37.07 | 5.16 | 11.45 | 45.52 |
CV (%) | 5.74 | 4.50 | 4.39 | 5.98 | 4.66 | 5.39 |
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