产品详情
简单介绍:
Human AQP3 (Aquaporin-3) Sandwich ELISA (STJE0002265)
详情介绍:
Applications: | ELISA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 0.19ng/mL |
Detection Limit: | 0.31~20ng/mL |
Short Description: | This AQP3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | AQP3 |
Gene ID: | 360 |
Uniprot ID: | AQP3_HUMAN |
Specificity: | This kit recognizes Human AQP-3 in samples. No significant cross-reactivity or interference between Human AQP-3 and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Function | Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. |
Protein Name | Aquaporin-3 Aqp-3 Aquaglyceroporin-3 |
Database Links | Reactome: R-HSA-432040 Reactome: R-HSA-432047 |
Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Basolateral Cell Membrane |
Alternative ELISA Names | Aquaporin-3 ELISA kit Aqp-3 ELISA kit Aquaglyceroporin-3 ELISA kit AQP3 ELISA kit |
Specificity | This kit recognizes Human AQP-3 in samples. No significant cross-reactivity or interference between Human AQP-3 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item Specifications Storage
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Sample Type Range (%) Average Recovery (%)
Serum(n=8) | 95-109 | 100 |
EDTA plasma (n=8) | 93-106 | 100 |
Cell culture media (n=8) | 85-97 | 90 |
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 1.06 | 2.77 | 7.89 | 1.12 | 2.59 | 7.86 |
Standard deviation | 0.06 | 0.14 | 0.38 | 0.06 | 0.15 | 0.32 |
CV (%) | 5.66 | 5.05 | 4.82 | 5.36 | 5.79 | 4.07 |
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