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  • 产品名称：Human AQP4 (Aquaporin-4) CLIA Kit (STJC0000286)

  • 产品型号：STJC0000286
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human AQP4 (Aquaporin-4) CLIA Kit (STJC0000286)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 46.88pg/mL
Detection Limit: 78.13~5000pg/mL
Short Description: This human AQP4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: AQP4
Gene ID: 361
Uniprot ID: AQP4_HUMAN
Specificity: This kit recognizes Human AQP-4 in samples. No significant cross-reactivity or interference between Human AQP-4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Detected in skeletal muscle. Detected in stomach, along the glandular base region of the fundic gland (at protein level). Detected in brain, lung and skeletal muscle, and at much lower levels in heart and ovary.
Post Translational Modifications Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamate increases conductance by 40%. Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not palmitoylated.
Function Forms a water-specific channel. Plays an important role in brain water homeostasis and in glymphatic solute transport. Required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability.
Protein Name Aquaporin-4
Aqp-4
Mercurial-Insensitive Water Channel
Miwc
Wch4
Database Links Reactome: R-HSA-432040
Reactome: R-HSA-432047
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Endosome Membrane
Sarcolemma
Cell Projection
Activation Of The Vasopressin Receptor Avpr1a Triggers Aqp4 Phosphorylation At Ser-180 And Promotes Its Internalization From The Cell Membrane
Detected On Brain Astrocyte Processes And Astrocyte Endfeet Close To Capillaries
Alternative CLIA Names Aquaporin-4 CLIA kit
Aqp-4 CLIA kit
Mercurial-Insensitive Water Channel CLIA kit
Miwc CLIA kit
Wch4 CLIA kit
AQP4 CLIA kit
Specificity This kit recognizes Human AQP-4 in samples. No significant cross-reactivity or interference between Human AQP-4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-107 100
EDTA plasma(n=8) 98-117 107
Cell culture media(n=8) 87-101 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 275.58 600.41 2436.64 289.73 552.47 2533.49
Standard deviation 29.05 57.58 224.66 32.62 48.62 250.82
CV (%) 10.54 9.59 9.22 11.26 8.80 9.90
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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