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  • 产品名称：Human BAG3 (BAG family molecular chaperone regulator 3) CLIA Kit (STJC0000244)

  • 产品型号：STJC0000244
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human BAG3 (BAG family molecular chaperone regulator 3) CLIA Kit (STJC0000244)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human BAG3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: BAG3
Gene ID: 9531
Uniprot ID: BAG3_HUMAN
Specificity: This kit recognizes Human BAG3 in samples. No significant cross-reactivity or interference between Human BAG3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70. Has anti-apoptotic activity. Plays a role in the HSF1 nucleocytoplasmic transport.
Protein Name Bag Family Molecular Chaperone Regulator 3
Bag-3
Bcl-2-Associated Athanogene 3
Bcl-2-Binding Protein Bis
Docking Protein Cair-1
Database Links Reactome: R-HSA-3371453
Cellular Localisation Nucleus
Cytoplasm
Colocalizes With Hsf1 To The Nucleus Upon Heat Stress
Alternative CLIA Names Bag Family Molecular Chaperone Regulator 3 CLIA kit
Bag-3 CLIA kit
Bcl-2-Associated Athanogene 3 CLIA kit
Bcl-2-Binding Protein Bis CLIA kit
Docking Protein Cair-1 CLIA kit
BAG3 CLIA kit
BIS CLIA kit
Specificity This kit recognizes Human BAG3 in samples. No significant cross-reactivity or interference between Human BAG3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-108 99
EDTA plasma(n=8) 88-103 95
Cell culture media(n=8) 99-113 107

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 95.00 180.60 975.47 93.66 179.48 1051.19
Standard deviation 8.06 13.17 69.26 8.80 17.01 79.79
CV (%) 8.48 7.29 7.10 9.40 9.48 7.59
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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