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  • 产品名称：Human BECN1 (Beclin-1) Sandwich ELISA (STJE0002319)

  • 产品型号：STJE0002319
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human BECN1 (Beclin-1) Sandwich ELISA (STJE0002319)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This BECN1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: BECN1
Gene ID: 8678
Uniprot ID: BECN1_HUMAN
Specificity: This kit recognizes Human BECN1 in samples. No significant cross-reactivity or interference between Human BECN1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy. In response to autophagic stimuli, phosphorylated at serine residues by AMPK in an ATG14-dependent manner, and this phosphorylation is critical for maximally efficient autophagy. Polyubiquitinated by NEDD4, both with 'Lys-11'- and 'Lys-63'-linkages. 'Lys-11'-linked polyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes. Polyubiquitinated at Lys-402 with 'Lys-48'-linkages. 'Lys-48'-linked polyubiquitination of Lys-402 leads to degradation. Deubiquitinated by ATXN3, leading to stabilization. Ubiquitinated at Lys-437 via 'Lys-63'-linkage by the DCX(AMBRA1) complex, thereby increasing the association between BECN1 and PIK3C3 to promote PIK3C3 activity. 'Lys-48'-linked ubiquitination by RNF216 leads to proteasomal degradation and autophagy inhibition. Proteolytically processed by caspases including CASP8 and CASP3.the C-terminal fragments lack autophagy-inducing capacity and are proposed to induce apoptosis. Thus the cleavage is proposed to be an determinant to switch from autophagy to apoptosis pathways affecting cellular homeostasis including viral infections and survival of tumor cells.
Function Plays a central role in autophagy. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. Protects against infection by a neurovirulent strain of Sindbis virus. May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis.it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.
Protein Name Beclin-1
Coiled-Coil Myosin-Like Bcl2-Interacting Protein
Protein Gt197 Cleaved Into - Beclin-1-C 35 Kda - Beclin-1-C 37 Kda
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-5689880
Reactome: R-HSA-9679504
Reactome: R-HSA-9694676
Reactome: R-HSA-9705671
Reactome: R-HSA-983170
Cellular Localisation Cytoplasm
Golgi Apparatus
Trans-Golgi Network Membrane
Peripheral Membrane Protein
Endosome Membrane
Endoplasmic Reticulum Membrane
Mitochondrion Membrane
Endosome
Cytoplasmic Vesicle
Autophagosome
Interaction With Atg14 Promotes Translocation To Autophagosomes
Expressed In Dendrites And Cell Bodies Of Cerebellar Purkinje Cells
Beclin-1-C 35 Kda: Mitochondrion
Nucleus
Beclin-1-C 37 Kda: Mitochondrion
Alternative ELISA Names Beclin-1 ELISA kit
Coiled-Coil Myosin-Like Bcl2-Interacting Protein ELISA kit
Protein Gt197 Cleaved Into - Beclin-1-C 35 Kda - Beclin-1-C 37 Kda ELISA kit
BECN1 ELISA kit
GT197 ELISA kit
Specificity This kit recognizes Human BECN1 in samples. No significant cross-reactivity or interference between Human BECN1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-111 101
EDTA plasma (n=8) 93-106 101
Cell culture media (n=8) 93-107 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.49 1.15 4.39 0.53 1.05 4.42
Standard deviation 0.03 0.06 0.16 0.03 0.06 0.18
CV (%) 6.12 5.22 3.64 5.66 5.71 4.07
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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