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  • 产品名称：Human Stem Cell Factor (mammalian) protein (Recombinant) (STJP000236)

  • 产品型号：STJP000236
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human Stem Cell Factor (mammalian) protein (Recombinant) (STJP000236)
详情介绍：
Host: CHO cells
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human Stem Cell Factor (mammalian)-protein was developed from cho cells. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 Mu m filtered solution in PBS pH7.0.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA
Immunoreactivity: The ED (50) was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤.0 ng/ml, corresponding to a specific activity of ≥ x units/mg. NA
Gene Symbol: KITLG
Gene ID: 4254
Uniprot ID: SCF_HUMAN
Immunogen Region: Mature chain
Immunogen: Optimized DNA sequence encoding Human SCF mature chain was expressed in HEK293 Cells. NA
Immunogen Sequence: EGICR NRVTNNVKDV TKLVANLPKD YMITLKYVPG MDVLPSHCWI SEMVVQLSDS LTDLLDKFSN ISEGLSNYSI IDKLVNIVDD LVECVKENSS KDLKKSFKSP EPRLFTPEEF FRIFNRSIDA FKDFVVASET SDCVVSSTLS PEKDSRVSVT KPFMLPPVAA NA
Post Translational Modifications A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain. Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97. A soluble form exists as a cleavage product of the extracellular domain.
Function Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Protein Name Kit Ligand
Mast Cell Growth Factor
Mgf
Stem Cell Factor
Scf
C-Kit Ligand Cleaved Into - Soluble Kit Ligand
Skitlg
Database Links Reactome: R-HSA-1257604 P21583-1
Reactome: R-HSA-1433557 P21583-1
Reactome: R-HSA-1433559 P21583-1
Reactome: R-HSA-2219530 P21583-1
Reactome: R-HSA-5673001 P21583-1
Reactome: R-HSA-6811558 P21583-1
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Cytoplasm
Cytoplasm
Cytoskeleton
Cell Membrane
Cell Projection
Lamellipodium
Filopodium
Soluble Kit Ligand: Secreted
Alternative Protein Names Kit Ligand protein
Mast Cell Growth Factor protein
Mgf protein
Stem Cell Factor protein
Scf protein
C-Kit Ligand Cleaved Into - Soluble Kit Ligand protein
Skitlg protein
KITLG protein
MGF protein
SCF protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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