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  • 产品名称：Human C4A (Complement C4-A) Sandwich ELISA (STJE0002045)

  • 产品型号：STJE0002045
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human C4A (Complement C4-A) Sandwich ELISA (STJE0002045)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.94ng/mL
Detection Limit: 1.56~100ng/mL
Short Description: This C4A Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: C4A
Gene ID: 720/721
Uniprot ID: CO4A_HUMAN
Specificity: This kit recognizes Human C4a in samples. No significant cross-reactivity or interference between Human C4a and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Prior to secretion, the single-chain precursor is enzymatically cleaved to yield non-identical chains alpha, beta and gamma. During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase. The proteolytic cleavages often are incomplete so that many structural forms can be found in plasma. N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Function Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
Protein Name Complement C4-A
Acidic Complement C4
C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 2 Cleaved Into - Complement C4 Beta Chain - Complement C4-A Alpha Chain - C4a Anaphylatoxin - C4b-A - C4d-A - Complement C4 Gamma Chain
Database Links Reactome: R-HSA-166663
Reactome: R-HSA-174577
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Reactome: R-HSA-977606
Cellular Localisation Secreted
Synapse
Cell Projection
Axon
Dendrite
Alternative ELISA Names Complement C4-A ELISA kit
Acidic Complement C4 ELISA kit
C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 2 Cleaved Into - Complement C4 Beta Chain - Complement C4-A Alpha Chain - C4a Anaphylatoxin - C4b-A - C4d-A - Complement C4 Gamma Chain ELISA kit
C4A ELISA kit
CO4 ELISA kit
CPAMD2 ELISA kit
Specificity This kit recognizes Human C4a in samples. No significant cross-reactivity or interference between Human C4a and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-100 95
EDTA plasma (n=8) 86-101 92
Cell culture media (n=8) 91-106 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 4.84 13.18 42.91 4.58 13.63 39.00
Standard deviation 0.27 0.70 1.77 0.23 0.75 1.56
CV (%) 5.58 5.31 4.12 5.02 5.50 4.00
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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