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  • 产品名称：Human CA2 (Carbonic anhydrase 2) CLIA Kit (STJC0000348)

  • 产品型号：STJC0000348
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CA2 (Carbonic anhydrase 2) CLIA Kit (STJC0000348)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.38ng/mL
Detection Limit: 0.63~40ng/mL
Short Description: This human CA2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CA2
Gene ID: 760
Uniprot ID: CAH2_HUMAN
Specificity: This kit recognizes Human CA2 in samples. No significant cross-reactivity or interference between Human CA2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Catalyzes the reversible hydration of carbon dioxide. Can also hydrate cyanamide to urea. Stimulates the chloride-bicarbonate exchange activity of SLC26A6. Essential for bone resorption and osteoclast differentiation. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption.
Protein Name Carbonic Anhydrase 2
Carbonate Dehydratase Ii
Carbonic Anhydrase C
Cac
Carbonic Anhydrase Ii
Ca-Ii
Cyanamide Hydratase Ca2
Database Links Reactome: R-HSA-1237044
Reactome: R-HSA-1247673
Reactome: R-HSA-1475029
Cellular Localisation Cytoplasm
Cell Membrane
Colocalized With Slc26a6 At The Surface Of The Cell Membrane In Order To Form A Bicarbonate Transport Metabolon
Displaced From The Cytosolic Surface Of The Cell Membrane By Pkc In Phorbol Myristate Acetate (Pma)-Induced Cells
Alternative CLIA Names Carbonic Anhydrase 2 CLIA kit
Carbonate Dehydratase Ii CLIA kit
Carbonic Anhydrase C CLIA kit
Cac CLIA kit
Carbonic Anhydrase Ii CLIA kit
Ca-Ii CLIA kit
Cyanamide Hydratase Ca2 CLIA kit
CA2 CLIA kit
Specificity This kit recognizes Human CA2 in samples. No significant cross-reactivity or interference between Human CA2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-106 101
EDTA plasma(n=8) 95-109 102
Cell culture media(n=8) 90-101 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.00 5.04 15.04 2.14 5.40 16.47
Standard deviation 0.17 0.47 1.51 0.25 0.57 1.15
CV (%) 8.50 9.33 10.04 11.68 10.56 6.98
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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