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  • 产品名称：Human CA9 (Carbonic anhydrase 9) CLIA Kit (STJC0000015)

  • 产品型号：STJC0000015
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CA9 (Carbonic anhydrase 9) CLIA Kit (STJC0000015)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pg/mL
Detection Limit: 12.5~800pg/mL
Short Description: This human CA9 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CA9
Gene ID: 768
Uniprot ID: CAH9_HUMAN
Specificity: This kit recognizes Human CA9 in samples. No significant cross-reactivity or interference between Human CA9 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Post Translational Modifications Asn-346 bears high-mannose type glycan structures.
Function Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
Protein Name Carbonic Anhydrase 9
Carbonate Dehydratase Ix
Carbonic Anhydrase Ix
Ca-Ix
Caix
Membrane Antigen Mn
P54/58n
Renal Cell Carcinoma-Associated Antigen G250
Rcc-Associated Antigen G250
Pmw1
Database Links Reactome: R-HSA-1234158
Reactome: R-HSA-1475029
Cellular Localisation Nucleus
Nucleolus
Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Microvillus Membrane
Found On The Surface Microvilli And In The Nucleus
Particularly In Nucleolus
Alternative CLIA Names Carbonic Anhydrase 9 CLIA kit
Carbonate Dehydratase Ix CLIA kit
Carbonic Anhydrase Ix CLIA kit
Ca-Ix CLIA kit
Caix CLIA kit
Membrane Antigen Mn CLIA kit
P54/58n CLIA kit
Renal Cell Carcinoma-Associated Antigen G250 CLIA kit
Rcc-Associated Antigen G250 CLIA kit
Pmw1 CLIA kit
CA9 CLIA kit
G250 CLIA kit
MN CLIA kit
Specificity This kit recognizes Human CA9 in samples. No significant cross-reactivity or interference between Human CA9 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-99 92
EDTA plasma(n=8) 89-104 97
Cell culture media(n=8) 94-107 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 39.20 76.13 270.93 35.47 81.46 249.33
Standard deviation 4.03 5.70 25.12 3.55 9.18 21.92
CV (%) 10.28 7.49 9.27 10.01 11.27 8.79
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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