Human CALR (Calreticulin) CLIA Kit (STJC0000338) STJC0000338|产品目录|代理商|上海玉博生物科技有限公司

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产品名称：Human CALR (Calreticulin) CLIA Kit (STJC0000338)
产品型号：STJC0000338
产品厂商：ST john's laboratory
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简单介绍：

Human CALR (Calreticulin) CLIA Kit (STJC0000338)

详情介绍：

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	18.75pg/mL
Detection Limit:	31.25~2000pg/mL
Short Description:	This human CALR kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	CALR
Gene ID:	811
Uniprot ID:	CALR_HUMAN
Specificity:	This kit recognizes Human CRT in samples. No significant cross-reactivity or interference between Human CRT and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy.
Protein Name	Calreticulin Crp55 Calregulin Endoplasmic Reticulum Resident Protein 60 Erp60 Hacbp Grp60
Database Links	Reactome: R-HSA-1236974 Reactome: R-HSA-168316 Reactome: R-HSA-3000480 Reactome: R-HSA-3000484 Reactome: R-HSA-381183 Reactome: R-HSA-901042 Reactome: R-HSA-983170
Cellular Localisation	Endoplasmic Reticulum Lumen Cytoplasm Cytosol Secreted Extracellular Space Extracellular Matrix Cell Surface Sarcoplasmic Reticulum Lumen Cytoplasmic Vesicle Secretory Vesicle Cortical Granule Cytolytic Granule Also Found In Cell Surface (T Cells) Cytosol And Extracellular Matrix During Oocyte Maturation And After Parthenogenetic Activation Accumulates In Cortical Granules In Pronuclear And Early Cleaved Embryos Localizes Weakly To Cytoplasm Around Nucleus And More Strongly In The Region Near The Cortex In Cortical Granules Of Non-Activated Oocytes Is Exocytosed During The Cortical Reaction In Response To Oocyte Activation
Alternative CLIA Names	Calreticulin CLIA kit Crp55 CLIA kit Calregulin CLIA kit Endoplasmic Reticulum Resident Protein 60 CLIA kit Erp60 CLIA kit Hacbp CLIA kit Grp60 CLIA kit CALR CLIA kit CRTC CLIA kit
Specificity	This kit recognizes Human CRT in samples. No significant cross-reactivity or interference between Human CRT and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	94-106	100
EDTA plasma(n=8)	89-104	95
Cell culture media(n=8)	95-111	101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	92.04	296.16	681.40	87.76	271.58	717.76
Standard deviation	9.48	24.85	62.21	7.29	24.28	63.81
CV (%)	10.30	8.39	9.13	8.31	8.94	8.89

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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产品名称：Human CALR (Calreticulin) CLIA Kit (STJC0000338)