产品详情
简单介绍：
Human ANGPTL4 (Angiopoietin-related protein 4) Sandwich ELISA (STJE0002139)
详情介绍：
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.94ng/mL |
| Detection Limit: | 1.56~100ng/mL |
| Short Description: | This ANGPTL4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | ANGPTL4 |
| Gene ID: | 51129 |
| Uniprot ID: | ANGL4_HUMAN |
| Specificity: | This kit recognizes Human ANGPTL4 in samples. No significant cross-reactivity or interference between Human ANGPTL4 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | N-glycosylated. ANGPTL4 N-terminal chain: Forms disulfide-linked dimers and tetramers. Cleaved into a smaller N-terminal chain and a larger chain that contains the fibrinogen C-terminal domain.both cleaved and uncleaved forms are detected in the extracellular space. The cleaved form is not present within the cell. |
| Function | Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. May also play a role in regulating glucose homeostasis and insulin sensitivity (Probable). Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro). Depending on context, may modulate tumor-related angiogenesis. ANGPTL4 N-terminal chain: Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. Has higher activity in LPL inactivation than the uncleaved protein. |
| Protein Name | Angiopoietin-Related Protein 4 Angiopoietin-Like Protein 4 Hepatic Fibrinogen/Angiopoietin-Related Protein Hfarp Cleaved Into - Angptl4 N-Terminal Chain - Angptl4 C-Terminal Chain |
| Database Links | Reactome: R-HSA-1989781 Reactome: R-HSA-381340 Reactome: R-HSA-8963889 Reactome: R-HSA-9762292 |
| Cellular Localisation | Secreted Extracellular Space Extracellular Matrix The Unprocessed Form Interacts With The Extracellular Matrix This May Constitute A Dynamic Reservoir A Regulatory Mechanism Of The Bioavailability Of Angptl4 (Probable) |
| Alternative ELISA Names | Angiopoietin-Related Protein 4 ELISA kit Angiopoietin-Like Protein 4 ELISA kit Hepatic Fibrinogen/Angiopoietin-Related Protein ELISA kit Hfarp Cleaved Into - Angptl4 N-Terminal Chain - Angptl4 C-Terminal Chain ELISA kit ANGPTL4 ELISA kit ARP4 ELISA kit HFARP ELISA kit PGAR ELISA kit PP1158 ELISA kit PSEC0166 ELISA kit UNQ171 ELISA kit PRO197 ELISA kit |
| Specificity | This kit recognizes Human ANGPTL4 in samples. No significant cross-reactivity or interference between Human ANGPTL4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 87-100 | 94 |
| EDTA plasma (n=8) | 91-102 | 96 |
| Cell culture media (n=8) | 88-98 | 93 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 4.73 | 12.85 | 40.94 | 4.48 | 12.46 | 40.23 |
| Standard deviation | 0.29 | 0.57 | 2.04 | 0.28 | 0.52 | 1.62 |
| CV (%) | 6.13 | 4.44 | 4.98 | 6.25 | 4.17 | 4.03 |
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