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  • 产品名称：Human ASK1 (Apoptosis Signal Regulating Kinase 1) Sandwich ELISA Kit (STJE0007335)

  • 产品型号：STJE0007335
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human ASK1 (Apoptosis Signal Regulating Kinase 1) Sandwich ELISA Kit (STJE0007335)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.107ng/mL
Detection Limit: 0.312-20ng/mL
Short Description: This ASK1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: MAP3K5
Gene ID: 4217
Uniprot ID: M3K5_HUMAN
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity Abundantly expressed in heart and pancreas.
Post Translational Modifications Phosphorylated at Thr-838 through autophosphorylation and by MAP3K6/ASK2 which leads to activation. Thr-838 is dephosphorylated by PPP5C. Ser-83 and Ser-1033 are inactivating phosphorylation sites, the former of which is phosphorylated by AKT1. Phosphorylated at Ser-966 which induces association of MAP3K5/ASK1 with the 14-3-3 family proteins and suppresses MAP3K5/ASK1 activity. Calcineurin (CN) dephosphorylates this site. Also dephosphorylated and activated by PGAM5. Phosphorylation at Ser-966 in response to oxidative stress is negatively regulated by PPIA/CYPA. Ubiquitinated. Tumor necrosis factor (TNF) induces TNFR2-dependent ubiquitination, leading to proteasomal degradation. Ubiquitinated by RC3H2 in a TRIM48-dependent manner. Methylation at Arg-78 and Arg-80 by PRMT1 promotes association of MAP3K5 with thioredoxin and negatively regulates MAP3K5 association with TRAF2, inhibiting MAP3K5 activation. Methylation is blocked by ubiquitination of PRMT1 by TRIM48.
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
Protein Name Mitogen-Activated Protein Kinase Kinase Kinase 5
Apoptosis Signal-Regulating Kinase 1
Ask-1
Mapk/Erk Kinase Kinase 5
Mek Kinase 5
Mekk 5
Database Links Reactome: R-HSA-2559580
Cellular Localisation Cytoplasm
Endoplasmic Reticulum
Interaction With 14-3-3 Proteins Alters The Distribution Of Map3k5/Ask1 And Restricts It To The Perinuclear Endoplasmic Reticulum Region
Alternative ELISA Names Mitogen-Activated Protein Kinase Kinase Kinase 5 ELISA kit
Apoptosis Signal-Regulating Kinase 1 ELISA kit
Ask-1 ELISA kit
Mapk/Erk Kinase Kinase 5 ELISA kit
Mek Kinase 5 ELISA kit
Mekk 5 ELISA kit
MAP3K5 ELISA kit
ASK1 ELISA kit
MAPKKK5 ELISA kit
MEKK5 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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