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  • 产品名称：Human CA12 (Carbonic anhydrase 12) CLIA Kit (STJC0000382)

  • 产品型号：STJC0000382
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CA12 (Carbonic anhydrase 12) CLIA Kit (STJC0000382)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This human CA12 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CA12
Gene ID: 771
Uniprot ID: CAH12_HUMAN
Specificity: This kit recognizes Human CA12 in samples. No significant cross-reactivity or interference between Human CA12 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat glands and bronchiolar epithelium.
Function Reversible hydration of carbon dioxide.
Protein Name Carbonic Anhydrase 12
Carbonate Dehydratase Xii
Carbonic Anhydrase Xii
Ca-Xii
Tumor Antigen Hom-Rcc-3.1.3
Database Links Reactome: R-HSA-1475029
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Cell Membrane
Alternative CLIA Names Carbonic Anhydrase 12 CLIA kit
Carbonate Dehydratase Xii CLIA kit
Carbonic Anhydrase Xii CLIA kit
Ca-Xii CLIA kit
Tumor Antigen Hom-Rcc-3.1.3 CLIA kit
CA12 CLIA kit
Specificity This kit recognizes Human CA12 in samples. No significant cross-reactivity or interference between Human CA12 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-102 93
EDTA plasma(n=8) 85-99 92
Cell culture media(n=8) 89-102 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 188.46 452.17 1616.46 183.05 457.51 1483.26
Standard deviation 23.75 50.91 148.39 17.52 48.36 101.01
CV (%) 12.60 11.26 9.18 9.57 10.57 6.81
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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