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  • 产品名称：Human CCL14 (C-C motif chemokine 14) CLIA Kit (STJC0000369)

  • 产品型号：STJC0000369
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CCL14 (C-C motif chemokine 14) CLIA Kit (STJC0000369)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pg/mL
Detection Limit: 12.5~800pg/mL
Short Description: This human CCL14 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CCL14
Gene ID: 6358
Uniprot ID: CCL14_HUMAN
Specificity: This kit recognizes Human CCL14 in samples. No significant cross-reactivity or interference between Human CCL14 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma.
Post Translational Modifications The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood. HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated.the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids.
Function Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induces intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and is inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes, eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.
Protein Name C-C Motif Chemokine 14
Chemokine Cc-1/Cc-3
Hcc-1/Hcc-3
Hcc-1(1-74
Ncc-2
Small-Inducible Cytokine A14 Cleaved Into - Hcc-1(3-74 - Hcc-1(4-74 - Hcc-1(9-74
Cellular Localisation Secreted
Alternative CLIA Names C-C Motif Chemokine 14 CLIA kit
Chemokine Cc-1/Cc-3 CLIA kit
Hcc-1/Hcc-3 CLIA kit
Hcc-1(1-74 CLIA kit
Ncc-2 CLIA kit
Small-Inducible Cytokine A14 Cleaved Into - Hcc-1(3-74 - Hcc-1(4-74 - Hcc-1(9-74 CLIA kit
CCL14 CLIA kit
NCC2 CLIA kit
SCYA14 CLIA kit
Specificity This kit recognizes Human CCL14 in samples. No significant cross-reactivity or interference between Human CCL14 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-103 95
EDTA plasma(n=8) 89-102 95
Cell culture media(n=8) 86-100 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 39.77 127.62 317.92 38.63 118.11 324.41
Standard deviation 3.47 11.29 22.00 3.64 9.73 26.80
CV (%) 8.73 8.85 6.92 9.42 8.24 8.26
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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