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  • 产品名称：Human CCL17 (C-C motif chemokine 17) Sandwich ELISA (STJE0001929)

  • 产品型号：STJE0001929
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CCL17 (C-C motif chemokine 17) Sandwich ELISA (STJE0001929)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This CCL17 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CCL17
Gene ID: 6361
Uniprot ID: CCL17_HUMAN
Specificity: This kit recognizes Human TARC in samples. No significant cross-reactivity or interference between Human TARC and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Chemokine, which displays chemotactic activity for T lymphocytes, preferentially Th2 cells, but not monocytes or granulocytes. Therefore plays an important role in a wide range of inflammatory and immunological processes. Acts by binding to CCR4 at T-cell surface. Mediates GM-CSF/CSF2-driven pain and inflammation. In the brain, required to maintain the typical, highly branched morphology of hippocampal microglia under homeostatic conditions. May be important for the appropriate adaptation of microglial morphology and synaptic plasticity to acute lipopolysaccharide (LPS)-induced neuroinflammation. Plays a role in wound healing, mainly by inducing fibroblast migration into the wound.
Protein Name C-C Motif Chemokine 17
Cc Chemokine Tarc
Small-Inducible Cytokine A17
Thymus And Activation-Regulated Chemokine
Database Links Reactome: R-HSA-380108
Cellular Localisation Secreted
Alternative ELISA Names C-C Motif Chemokine 17 ELISA kit
Cc Chemokine Tarc ELISA kit
Small-Inducible Cytokine A17 ELISA kit
Thymus And Activation-Regulated Chemokine ELISA kit
CCL17 ELISA kit
SCYA17 ELISA kit
TARC ELISA kit
Specificity This kit recognizes Human TARC in samples. No significant cross-reactivity or interference between Human TARC and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-100 92
EDTA plasma (n=8) 90-104 96
Cell culture media (n=8) 90-102 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 95.26 217.44 679.06 102.11 209.10 668.22
Standard deviation 6.47 12.24 21.12 6.98 10.69 22.25
CV (%) 6.79 5.63 3.11 6.84 5.11 3.33
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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