Name: Human CCL4 (C-C motif chemokine 4) CLIA Kit (STJC0000022)
Brand: ST john's laboratory
SKU: STJC0000022

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产品名称：Human CCL4 (C-C motif chemokine 4) CLIA Kit (STJC0000022)
产品型号：STJC0000022
产品厂商：ST john's laboratory
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简单介绍：

Human CCL4 (C-C motif chemokine 4) CLIA Kit (STJC0000022)

详情介绍：

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	9.38pg/mL
Detection Limit:	15.63~1000pg/mL
Short Description:	This human CCL4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	CCL4
Gene ID:	388372/6351
Uniprot ID:	CCL4_HUMAN
Specificity:	This kit recognizes Human MIP-1 Beta in samples. No significant cross-reactivity or interference between Human MIP-1 Beta and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
Function	Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
Protein Name	C-C Motif Chemokine 4 G-26 T-Lymphocyte-Secreted Protein Hc21 Lymphocyte Activation Gene 1 Protein Lag-1 Mip-1-Beta(1-69 Macrophage Inflammatory Protein 1-Beta Mip-1-Beta Pat 744 Protein H400 Sis-Gamma Small-Inducible Cytokine A4 T-Cell Activation Protein 2 Act-2 Cleaved Into - Mip-1-Beta(3-69
Database Links	Reactome: R-HSA-380108 Reactome: R-HSA-418594 Reactome: R-HSA-6783783
Cellular Localisation	Secreted
Alternative CLIA Names	C-C Motif Chemokine 4 CLIA kit G-26 T-Lymphocyte-Secreted Protein CLIA kit Hc21 CLIA kit Lymphocyte Activation Gene 1 Protein CLIA kit Lag-1 CLIA kit Mip-1-Beta(1-69 CLIA kit Macrophage Inflammatory Protein 1-Beta CLIA kit Mip-1-Beta CLIA kit Pat 744 CLIA kit Protein H400 CLIA kit Sis-Gamma CLIA kit Small-Inducible Cytokine A4 CLIA kit T-Cell Activation Protein 2 CLIA kit Act-2 Cleaved Into - Mip-1-Beta(3-69 CLIA kit CCL4 CLIA kit LAG1 CLIA kit MIP1B CLIA kit SCYA4 CLIA kit
Specificity	This kit recognizes Human MIP-1β in samples. No significant cross-reactivity or interference between Human MIP-1β and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	93-108	99
EDTA plasma(n=8)	100-114	106
Cell culture media(n=8)	91-103	98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	46.97	153.49	357.66	45.28	143.34	348.90
Standard deviation	4.87	18.27	30.26	5.48	15.84	23.93
CV (%)	10.37	11.90	8.46	12.10	11.05	6.86

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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产品名称：Human CCL4 (C-C motif chemokine 4) CLIA Kit (STJC0000022)