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  • 产品名称：Human CCL4 (C-C motif chemokine 4) CLIA Kit (STJC0000022)

  • 产品型号：STJC0000022
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CCL4 (C-C motif chemokine 4) CLIA Kit (STJC0000022)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human CCL4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CCL4
Gene ID: 388372/6351
Uniprot ID: CCL4_HUMAN
Specificity: This kit recognizes Human MIP-1 Beta in samples. No significant cross-reactivity or interference between Human MIP-1 Beta and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
Function Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
Protein Name C-C Motif Chemokine 4
G-26 T-Lymphocyte-Secreted Protein
Hc21
Lymphocyte Activation Gene 1 Protein
Lag-1
Mip-1-Beta(1-69
Macrophage Inflammatory Protein 1-Beta
Mip-1-Beta
Pat 744
Protein H400
Sis-Gamma
Small-Inducible Cytokine A4
T-Cell Activation Protein 2
Act-2 Cleaved Into - Mip-1-Beta(3-69
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-418594
Reactome: R-HSA-6783783
Cellular Localisation Secreted
Alternative CLIA Names C-C Motif Chemokine 4 CLIA kit
G-26 T-Lymphocyte-Secreted Protein CLIA kit
Hc21 CLIA kit
Lymphocyte Activation Gene 1 Protein CLIA kit
Lag-1 CLIA kit
Mip-1-Beta(1-69 CLIA kit
Macrophage Inflammatory Protein 1-Beta CLIA kit
Mip-1-Beta CLIA kit
Pat 744 CLIA kit
Protein H400 CLIA kit
Sis-Gamma CLIA kit
Small-Inducible Cytokine A4 CLIA kit
T-Cell Activation Protein 2 CLIA kit
Act-2 Cleaved Into - Mip-1-Beta(3-69 CLIA kit
CCL4 CLIA kit
LAG1 CLIA kit
MIP1B CLIA kit
SCYA4 CLIA kit
Specificity This kit recognizes Human MIP-1β in samples. No significant cross-reactivity or interference between Human MIP-1β and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-108 99
EDTA plasma(n=8) 100-114 106
Cell culture media(n=8) 91-103 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 46.97 153.49 357.66 45.28 143.34 348.90
Standard deviation 4.87 18.27 30.26 5.48 15.84 23.93
CV (%) 10.37 11.90 8.46 12.10 11.05 6.86
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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