产品详情
简单介绍:
Human CCN2 (CCN family member 2) CLIA Kit (STJC0000421)
详情介绍:
Applications: | CLIA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 9.38pg/mL |
Detection Limit: | 15.63~1000pg/mL |
Short Description: | This human CCN2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Chemiluminescence |
Gene Symbol: | CCN2 |
Gene ID: | 1490 |
Uniprot ID: | CCN2_HUMAN |
Specificity: | This kit recognizes Human CTGF in samples. No significant cross-reactivity or interference between Human CTGF and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Tissue Specificity | Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested. |
Function | Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. |
Protein Name | Ccn Family Member 2 Cellular Communication Network Factor 2 Connective Tissue Growth Factor Hypertrophic Chondrocyte-Specific Protein 24 Insulin-Like Growth Factor-Binding Protein 8 Ibp-8 Igf-Binding Protein 8 Igfbp-8 |
Database Links | Reactome: R-HSA-2032785 Reactome: R-HSA-8951671 |
Cellular Localisation | Secreted Extracellular Space Extracellular Matrix |
Alternative CLIA Names | Ccn Family Member 2 CLIA kit Cellular Communication Network Factor 2 CLIA kit Connective Tissue Growth Factor CLIA kit Hypertrophic Chondrocyte-Specific Protein 24 CLIA kit Insulin-Like Growth Factor-Binding Protein 8 CLIA kit Ibp-8 CLIA kit Igf-Binding Protein 8 CLIA kit Igfbp-8 CLIA kit CCN2 CLIA kit CTGF CLIA kit HCS24 CLIA kit IGFBP8 CLIA kit |
Specificity | This kit recognizes Human CTGF in samples. No significant cross-reactivity or interference between Human CTGF and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item Specifications Storage
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Sample Type Range (%) Average Recovery (%)
Serum(n=8) | 96-109 | 102 |
EDTA plasma(n=8) | 91-105 | 97 |
Cell culture media(n=8) | 98-110 | 103 |
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 51.99 | 107.29 | 487.85 | 52.58 | 96.98 | 520.00 |
Standard deviation | 4.52 | 8.13 | 44.30 | 5.99 | 8.84 | 44.67 |
CV (%) | 8.69 | 7.58 | 9.08 | 11.39 | 9.12 | 8.59 |
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