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  • 产品名称：Human CD23 (Receptor 2 for the Fc Region of Immunoglobulin E) Sandwich ELISA (STJE0019881)

  • 产品型号：STJE0019881
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CD23 (Receptor 2 for the Fc Region of Immunoglobulin E) Sandwich ELISA (STJE0019881)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 46.32 pg/mL
Detection Limit: 78.13-5000 pg/mL
Short Description: This CD23 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: FCER2
Gene ID: 2208
Uniprot ID: FCER2_HUMAN
Specificity: This kit recognizes Human CD23 in samples. No significant cross-reactivity or interference between Human CD23 and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Post Translational Modifications N- and O-glycosylated. The secreted form sCD23 is produced by ADAM10-mediated ectodomain shedding.
Function Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B cells. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, upon IgE binding and antigen cross-linking induces intracellular killing of parasites through activation of L-Arginine-nitric oxide pathway.
Protein Name Low Affinity Immunoglobulin Epsilon Fc Receptor
Blast-2
C-Type Lectin Domain Family 4 Member J
Fc-Epsilon-Rii
Immunoglobulin E-Binding Factor
Lymphocyte Ige Receptor
Cd Antigen Cd23 Cleaved Into - Low Affinity Immunoglobulin Epsilon Fc Receptor Membrane-Bound Form - Low Affinity Immunoglobulin Epsilon Fc Receptor Soluble Form
Database Links Reactome: R-HSA-2197563
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Lipid-Anchor
Secreted
Also Exists As A Soluble Excreted Form
Scd23
Alternative ELISA Names Low Affinity Immunoglobulin Epsilon Fc Receptor ELISA kit
Blast-2 ELISA kit
C-Type Lectin Domain Family 4 Member J ELISA kit
Fc-Epsilon-Rii ELISA kit
Immunoglobulin E-Binding Factor ELISA kit
Lymphocyte Ige Receptor ELISA kit
Cd Antigen Cd23 Cleaved Into - Low Affinity Immunoglobulin Epsilon Fc Receptor Membrane-Bound Form - Low Affinity Immunoglobulin Epsilon Fc Receptor Soluble Form ELISA kit
FCER2 ELISA kit
CD23A ELISA kit
CLEC4J ELISA kit
FCE2 ELISA kit
IGEBF ELISA kit
Specificity This kit recognizes Human CD23 in samples. No significant cross-reactivity or interference between Human CD23 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-103 95
EDTA plasma (n=8) 94-111 102
Cell culture media (n=8) 87-100 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 254.15 496.51 2391.82 240.95 539.50 2433.85
Standard deviation 13.77 19.96 74.62 16.87 30.75 77.88
CV (%) 5.42 4.02 3.12 7.00 5.70 3.20
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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