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  • 产品名称：Human CDH5 (Cadherin-5) Sandwich ELISA (STJE0004115)

  • 产品型号：STJE0004115
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CDH5 (Cadherin-5) Sandwich ELISA (STJE0004115)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This CDH5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CDH5
Gene ID: 1003
Uniprot ID: CADH5_HUMAN
Specificity: This kit recognizes Human VE-Cadherin in samples. No significant cross-reactivity or interference between Human VE-Cadherin and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. O-glycosylated.
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.
Protein Name Cadherin-5
7b4 Antigen
Vascular Endothelial Cadherin
Ve-Cadherin
Cd Antigen Cd144
Database Links Reactome: R-HSA-418990
Reactome: R-HSA-5218920
Cellular Localisation Cell Junction
Cell Membrane
Single-Pass Type I Membrane Protein
Found At Cell-Cell Boundaries And Probably At Cell-Matrix Boundaries
Krit1 And Cdh5 Reciprocally Regulate Their Localization To Endothelial Cell-Cell Junctions
Alternative ELISA Names Cadherin-5 ELISA kit
7b4 Antigen ELISA kit
Vascular Endothelial Cadherin ELISA kit
Ve-Cadherin ELISA kit
Cd Antigen Cd144 ELISA kit
CDH5 ELISA kit
Specificity This kit recognizes Human VE-Cadherin in samples. No significant cross-reactivity or interference between Human VE-Cadherin and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-103 100
EDTA plasma (n=8) 89-102 96
Cell culture media (n=8) 91-101 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 95.09 238.90 906.46 99.60 241.36 993.71
Standard deviation 4.94 10.75 42.42 5.45 11.54 46.90
CV (%) 5.20 4.50 4.68 5.47 4.78 4.72
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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