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  • 产品名称：RUNX1/AML1 Positive Control peptide (STJ505107)

  • 产品型号：STJ505107-5
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
RUNX1/AML1 Positive Control peptide (STJ505107)
详情介绍：
Applications: WB
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: RUNX1/AML1 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications.
Formulation: Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Dilution Range: WB: 1:500
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: RUNX1
Gene ID: 861
Uniprot ID: RUNX1_HUMAN
Tissue Specificity Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.
Post Translational Modifications Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with KAT6A. Methylated. Phosphorylated in Ser-249 Thr-273 and Ser-276 by HIPK2 when associated with CBFB and DNA. This phosphorylation promotes subsequent EP300 phosphorylation.
Function Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). Essential for the development of normal hematopoiesis. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter. Inhibits KAT6B-dependent transcriptional activation. Involved in lineage commitment of immature T cell precursors. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells. Positively regulates the expression of RORC in T-helper 17 cells. Isoform AML-1G shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation. Isoform AML-1L interferes with the transactivation activity of RUNX1.
Peptide Name Runt-Related Transcription Factor 1
Acute Myeloid Leukemia 1 Protein
Core-Binding Factor Subunit Alpha-2
Cbf-Alpha-2
Oncogene Aml-1
Polyomavirus Enhancer-Binding Protein 2 Alpha B Subunit
Pea2-Alpha B
Pebp2-Alpha B
Sl3-3 Enhancer Factor 1 Alpha B Subunit
Sl3/Akv Core-Binding Factor Alpha B Subunit
Database Links Reactome: R-HSA-1912408
Reactome: R-HSA-549127
Reactome: R-HSA-8877330
Reactome: R-HSA-8931987
Reactome: R-HSA-8934593
Reactome: R-HSA-8935964
Reactome: R-HSA-8936459
Reactome: R-HSA-8939236
Reactome: R-HSA-8939242
Reactome: R-HSA-8939243
Reactome: R-HSA-8939245
Reactome: R-HSA-8939246
Reactome: R-HSA-8939247
Reactome: R-HSA-8939256
Reactome: R-HSA-8941333
Reactome: R-HSA-8951936
Reactome: R-HSA-9018519
Reactome: R-HSA-9616222
Reactome: R-HSA-9692916
Cellular Localisation Nucleus
Alternative Peptide Names Runt-Related Transcription Factor 1 protein
Acute Myeloid Leukemia 1 Protein protein
Core-Binding Factor Subunit Alpha-2 protein
Cbf-Alpha-2 protein
Oncogene Aml-1 protein
Polyomavirus Enhancer-Binding Protein 2 Alpha B Subunit protein
Pea2-Alpha B protein
Pebp2-Alpha B protein
Sl3-3 Enhancer Factor 1 Alpha B Subunit protein
Sl3/Akv Core-Binding Factor Alpha B Subunit protein
RUNX1 protein
AML1 protein
CBFA2 protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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