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  • 产品名称：Human CSF1 (Macrophage colony-stimulating factor 1) CLIA Kit (STJC0000088)

  • 产品型号：STJC0000088
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CSF1 (Macrophage colony-stimulating factor 1) CLIA Kit (STJC0000088)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pg/mL
Detection Limit: 12.5~800pg/mL
Short Description: This human CSF1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CSF1
Gene ID: 1435
Uniprot ID: CSF1_HUMAN
Specificity: This kit recognizes Human MCSF in samples. No significant cross-reactivity or interference between Human MCSF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications N-glycosylated. Isoform 1: N-glycosylated. Isoform 3: N-glycosylated. O-glycosylated.contains chondroitin sulfate. O-glycosylated with core 1 or possibly core 8 glycans. Isoform 1: O-glycosylated.
Function Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
Protein Name Macrophage Colony-Stimulating Factor 1
Csf-1
M-Csf
Mcsf
Lanimostim
Proteoglycan Macrophage Colony-Stimulating Factor
Pg-M-Csf Cleaved Into - Processed Macrophage Colony-Stimulating Factor 1 - Macrophage Colony-Stimulating Factor 1 43 Kda Subunit
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-449836
Reactome: R-HSA-6783783
Reactome: R-HSA-8957275
Reactome: R-HSA-9680350
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Processed Macrophage Colony-Stimulating Factor 1: Secreted
Extracellular Space
Alternative CLIA Names Macrophage Colony-Stimulating Factor 1 CLIA kit
Csf-1 CLIA kit
M-Csf CLIA kit
Mcsf CLIA kit
Lanimostim CLIA kit
Proteoglycan Macrophage Colony-Stimulating Factor CLIA kit
Pg-M-Csf Cleaved Into - Processed Macrophage Colony-Stimulating Factor 1 - Macrophage Colony-Stimulating Factor 1 43 Kda Subunit CLIA kit
CSF1 CLIA kit
Specificity This kit recognizes Human MCSF in samples. No significant cross-reactivity or interference between Human MCSF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-107 98
EDTA plasma(n=8) 87-101 94
Cell culture media(n=8) 92-109 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 37.28 71.87 375.90 39.45 68.71 338.75
Standard deviation 4.82 7.70 41.54 3.45 7.72 30.32
CV (%) 12.93 10.71 11.05 8.75 11.24 8.95
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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