产品详情
简单介绍：
Human CSF1 (Macrophage colony-stimulating factor 1) Sandwich ELISA (STJE0001987)
详情介绍：
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description: | This CSF1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | CSF1 |
| Gene ID: | 1435 |
| Uniprot ID: | CSF1_HUMAN |
| Specificity: | This kit recognizes Human MCSF in samples. No significant cross-reactivity or interference between Human MCSF and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. |
| Protein Name | Macrophage Colony-Stimulating Factor 1 Csf-1 M-Csf Mcsf Lanimostim Proteoglycan Macrophage Colony-Stimulating Factor Pg-M-Csf Cleaved Into - Processed Macrophage Colony-Stimulating Factor 1 - Macrophage Colony-Stimulating Factor 1 43 Kda Subunit |
| Database Links | Reactome: R-HSA-381426 Reactome: R-HSA-449836 Reactome: R-HSA-6783783 Reactome: R-HSA-8957275 Reactome: R-HSA-9680350 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Processed Macrophage Colony-Stimulating Factor 1: Secreted Extracellular Space |
| Alternative ELISA Names | Macrophage Colony-Stimulating Factor 1 ELISA kit Csf-1 ELISA kit M-Csf ELISA kit Mcsf ELISA kit Lanimostim ELISA kit Proteoglycan Macrophage Colony-Stimulating Factor ELISA kit Pg-M-Csf Cleaved Into - Processed Macrophage Colony-Stimulating Factor 1 - Macrophage Colony-Stimulating Factor 1 43 Kda Subunit ELISA kit CSF1 ELISA kit |
| Specificity | This kit recognizes Human MCSF in samples. No significant cross-reactivity or interference between Human MCSF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 85-96 | 91 |
| EDTA plasma (n=8) | 85-98 | 90 |
| Cell culture media (n=8) | 85-97 | 92 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 94.07 | 270.27 | 679.52 | 100.99 | 263.75 | 714.11 |
| Standard deviation | 5.21 | 12.19 | 35.74 | 5.59 | 11.34 | 31.85 |
| CV (%) | 5.54 | 4.51 | 5.26 | 5.54 | 4.30 | 4.46 |
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