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  • 产品名称：Human CTSK (Cathepsin K) Sandwich ELISA (STJE0002389)

  • 产品型号：STJE0002389
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CTSK (Cathepsin K) Sandwich ELISA (STJE0002389)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This CTSK Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CTSK
Gene ID: 1513
Uniprot ID: CATK_HUMAN
Specificity: This kit recognizes Human CTSK in samples. No significant cross-reactivity or interference between Human CTSK and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Thiol protease involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen.
Protein Name Cathepsin K
Cathepsin O
Cathepsin O2
Cathepsin X
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-1679131
Reactome: R-HSA-2132295
Reactome: R-HSA-8939242
Cellular Localisation Lysosome
Secreted
Apical Cell Membrane
Peripheral Membrane Protein
Extracellular Side
Localizes To The Lumen Of Thyroid Follicles And To The Apical Membrane Of Thyroid Epithelial Cells
Alternative ELISA Names Cathepsin K ELISA kit
Cathepsin O ELISA kit
Cathepsin O2 ELISA kit
Cathepsin X ELISA kit
CTSK ELISA kit
CTSO ELISA kit
CTSO2 ELISA kit
Specificity This kit recognizes Human CTSK in samples. No significant cross-reactivity or interference between Human CTSK and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-97 92
EDTA plasma (n=8) 88-104 95
Cell culture media (n=8) 94-107 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.46 1.57 4.41 0.48 1.52 4.13
Standard deviation 0.03 0.08 0.17 0.03 0.07 0.16
CV (%) 6.52 5.10 3.85 6.25 4.61 3.87
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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