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  • 产品名称：Human CUBN (Cubilin) Sandwich ELISA (STJE0003735)

  • 产品型号：STJE0003735
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CUBN (Cubilin) Sandwich ELISA (STJE0003735)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47ng/mL
Detection Limit: 0.78~50ng/mL
Short Description: This CUBN Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CUBN
Gene ID: 8029
Uniprot ID: CUBN_HUMAN
Specificity: This kit recognizes Human CUBN in samples. No significant cross-reactivity or interference between Human CUBN and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications The precursor is cleaved by a trans-Golgi proteinase furin, removing a propeptide. N-glycosylated.
Function Endocytic receptor which plays a role in lipoprotein, vitamin and iron metabolism by facilitating their uptake. Acts together with LRP2 to mediate endocytosis of high-density lipoproteins, GC, hemoglobin, ALB, TF and SCGB1A1. Acts together with AMN to mediate endocytosis of the CBLIF-cobalamin complex. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the CBLIF-cobalamin complex. Ligand binding requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface.
Protein Name Cubilin
460 Kda Receptor
Intestinal Intrinsic Factor Receptor
Intrinsic Factor-Cobalamin Receptor
Intrinsic Factor-Vitamin B12 Receptor
Database Links Reactome: R-HSA-196791
Reactome: R-HSA-3359462
Reactome: R-HSA-3359463
Reactome: R-HSA-8964011
Reactome: R-HSA-9758881
Cellular Localisation Apical Cell Membrane
Peripheral Membrane Protein
Cell Membrane
Membrane
Coated Pit
Endosome
Lysosome Membrane
Lacks A Transmembrane Domain And Depends On Interaction With Amn For Location At The Plasma Membrane
Colocalizes With Amn And Lrp2 In The Endocytotic Apparatus Of Epithelial Cells
Alternative ELISA Names Cubilin ELISA kit
460 Kda Receptor ELISA kit
Intestinal Intrinsic Factor Receptor ELISA kit
Intrinsic Factor-Cobalamin Receptor ELISA kit
Intrinsic Factor-Vitamin B12 Receptor ELISA kit
CUBN ELISA kit
IFCR ELISA kit
Specificity This kit recognizes Human CUBN in samples. No significant cross-reactivity or interference between Human CUBN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-106 101
EDTA plasma (n=8) 88-100 93
Cell culture media (n=8) 95-108 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.35 5.20 17.41 2.41 4.70 18.84
Standard deviation 0.16 0.28 0.87 0.16 0.27 0.69
CV (%) 6.81 5.38 5.00 6.64 5.74 3.66
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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