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  • 产品名称：Human CYP1A1 (Cytochrome P450 1A1) Sandwich ELISA (STJE0004048)

  • 产品型号：STJE0004048
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CYP1A1 (Cytochrome P450 1A1) Sandwich ELISA (STJE0004048)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This CYP1A1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CYP1A1
Gene ID: 1543
Uniprot ID: CP1A1_HUMAN
Specificity: This kit recognizes Human CYP1A1 in samples. No significant cross-reactivity or interference between Human CYP1A1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15-alpha and C16-alpha positions. Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation. Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system. Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer. May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent).
Protein Name Cytochrome P450 1a1
Cypia1
Cytochrome P450 Form 6
Cytochrome P450-C
Cytochrome P450-P1
Hydroperoxy Icosatetraenoate Dehydratase
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-211981
Reactome: R-HSA-2142670
Reactome: R-HSA-2142816
Reactome: R-HSA-9018681
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Mitochondrion Inner Membrane
Microsome Membrane
Cytoplasm
Alternative ELISA Names Cytochrome P450 1a1 ELISA kit
Cypia1 ELISA kit
Cytochrome P450 Form 6 ELISA kit
Cytochrome P450-C ELISA kit
Cytochrome P450-P1 ELISA kit
Hydroperoxy Icosatetraenoate Dehydratase ELISA kit
CYP1A1 ELISA kit
Specificity This kit recognizes Human CYP1A1 in samples. No significant cross-reactivity or interference between Human CYP1A1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-104 96
EDTA plasma (n=8) 91-102 97
Cell culture media (n=8) 83-99 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.84 2.26 9.41 0.82 2.25 8.36
Standard deviation 0.05 0.11 0.44 0.05 0.11 0.35
CV (%) 5.95 4.87 4.68 6.10 4.89 4.19
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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