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  • 产品名称：Human CYP1B1 (Cytochrome P450 1B1) CLIA Kit (STJC0000229)

  • 产品型号：STJC0000229
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CYP1B1 (Cytochrome P450 1B1) CLIA Kit (STJC0000229)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human CYP1B1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CYP1B1
Gene ID: 1545
Uniprot ID: CP1B1_HUMAN
Specificity: This kit recognizes Human CYP1B1 in samples. No significant cross-reactivity or interference between Human CYP1B1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in heart, brain, lung, skeletal muscle, kidney, spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood leukocytes. Expressed in retinal endothelial cells and umbilical vein endothelial cells (at protein level).
Function A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Exhibits catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2- and 4-hydroxy E1 and E2. Displays a predominant hydroxylase activity toward E2 at the C-4 position. Metabolizes testosterone and progesterone to B or D ring hydroxylated metabolites. May act as a major enzyme for all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EpETrE) regioisomers, 8,9-, 11,12-, and 14,15- EpETrE, that function as lipid mediators in the vascular system. Additionally, displays dehydratase activity toward oxygenated eicosanoids hydroperoxyeicosatetraenoates (HpETEs). This activity is independent of cytochrome P450 reductase, NADPH, and O2. Also involved in the oxidative metabolism of xenobiotics, particularly converting polycyclic aromatic hydrocarbons and heterocyclic aryl amines procarcinogens to DNA-damaging products. Plays an important role in retinal vascular development. Under hyperoxic O2 conditions, promotes retinal angiogenesis and capillary morphogenesis, likely by metabolizing the oxygenated products generated during the oxidative stress. Also, contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression.
Protein Name Cytochrome P450 1b1
Cypib1
Hydroperoxy Icosatetraenoate Dehydratase
Database Links Reactome: R-HSA-211976
Reactome: R-HSA-2142670
Reactome: R-HSA-2142816
Reactome: R-HSA-5579000
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Microsome Membrane
Mitochondrion
Located Primarily In Endoplasmic Reticulum
Upon Treatment With 2
3
7
8-Tetrachlorodibenzo-P-Dioxin (Tcdd)
Cyp1b1 Is Also Targeted To Mitochondria
Alternative CLIA Names Cytochrome P450 1b1 CLIA kit
Cypib1 CLIA kit
Hydroperoxy Icosatetraenoate Dehydratase CLIA kit
CYP1B1 CLIA kit
Specificity This kit recognizes Human CYP1B1 in samples. No significant cross-reactivity or interference between Human CYP1B1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-103 95
EDTA plasma(n=8) 84-99 90
Cell culture media(n=8) 85-100 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 106.19 291.22 905.74 115.25 310.56 945.08
Standard deviation 9.33 28.48 57.33 13.05 36.71 91.86
CV (%) 8.79 9.78 6.33 11.32 11.82 9.72
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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