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  • 产品名称：Human DMD (Dystrophin) CLIA Kit (STJC0000638)

  • 产品型号：STJC0000638
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human DMD (Dystrophin) CLIA Kit (STJC0000638)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human DMD kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: DMD
Gene ID: 1756
Uniprot ID: DMD_HUMAN
Specificity: This kit recognizes Human DMD in samples. No significant cross-reactivity or interference between Human DMD and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma. Expressed in brain, muscle, kidney, lung and testis. Most tissues contain transcripts of multiple isoforms. Isoform 15: Only isoform to be detected in heart and liver and is also expressed in brain, testis and hepatoma cells.
Function Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission.
Protein Name Dystrophin
Database Links Reactome: R-HSA-3000171
Reactome: R-HSA-390522
Cellular Localisation Cell Membrane
Sarcolemma
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Postsynaptic Cell Membrane
In Muscle Cells
Sarcolemma Localization Requires The Presence Of Ank2
While Localization To Costameres Requires The Presence Of Ank3
Localizes To Neuromuscular Junctions (Nmjs)
In Adult Muscle
Nmj Localization Depends Upon Ank2 Presence
But Not In Newborn Animals
Alternative CLIA Names Dystrophin CLIA kit
DMD CLIA kit
Specificity This kit recognizes Human DMD in samples. No significant cross-reactivity or interference between Human DMD and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 97-113 105
EDTA plasma(n=8) 100-114 108
Cell culture media(n=8) 90-106 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 104.54 224.07 923.09 105.32 227.03 876.23
Standard deviation 11.04 23.17 92.03 9.26 21.61 95.42
CV (%) 10.56 10.34 9.97 8.79 9.52 10.89
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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