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  • 产品名称：Human DNASE1 (Deoxyribonuclease-1) Sandwich ELISA (STJE0003397)

  • 产品型号：STJE0003397
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human DNASE1 (Deoxyribonuclease-1) Sandwich ELISA (STJE0003397)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This DNASE1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: DNASE1
Gene ID: 1773
Uniprot ID: DNAS1_HUMAN
Specificity: This kit recognizes Human DNase-1 in samples. No significant cross-reactivity or interference between Human DNase-1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
Protein Name Deoxyribonuclease-1
Deoxyribonuclease I
Dnase I
Dornase Alfa
Cellular Localisation Secreted
Zymogen Granule
Nucleus Envelope
Secretory Protein
Stored In Zymogen Granules And Found In The Nuclear Envelope
Alternative ELISA Names Deoxyribonuclease-1 ELISA kit
Deoxyribonuclease I ELISA kit
Dnase I ELISA kit
Dornase Alfa ELISA kit
DNASE1 ELISA kit
DNL1 ELISA kit
DRNI ELISA kit
Specificity This kit recognizes Human DNase-Ⅰ in samples. No significant cross-reactivity or interference between Human DNase-Ⅰ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-103 95
EDTA plasma (n=8) 93-108 100
Cell culture media (n=8) 85-99 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.53 1.21 3.89 0.52 1.24 3.97
Standard deviation 0.04 0.07 0.15 0.03 0.06 0.16
CV (%) 7.55 5.79 3.86 5.77 4.84 4.03
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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