产品详情
简单介绍:
Human ECM1 (Extracellular matrix protein 1) CLIA Kit (STJC0000879)
详情介绍:
Applications: | CLIA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 7.5pg/mL |
Detection Limit: | 12.5~800pg/mL |
Short Description: | This human ECM1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Chemiluminescence |
Gene Symbol: | ECM1 |
Gene ID: | 1893 |
Uniprot ID: | ECM1_HUMAN |
Specificity: | This kit recognizes Human ECM1 in samples. No significant cross-reactivity or interference between Human ECM1 and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Tissue Specificity | Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin.wide expression is observed throughout the dermis with minimal expression in the epidermis. |
Function | Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. |
Protein Name | Extracellular Matrix Protein 1 Secretory Component P85 |
Database Links | Reactome: R-HSA-114608 |
Cellular Localisation | Secreted Extracellular Space Extracellular Matrix |
Alternative CLIA Names | Extracellular Matrix Protein 1 CLIA kit Secretory Component P85 CLIA kit ECM1 CLIA kit |
Specificity | This kit recognizes Human ECM1 in samples. No significant cross-reactivity or interference between Human ECM1 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item Specifications Storage
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Sample Type Range (%) Average Recovery (%)
Serum(n=8) | 88-101 | 94 |
EDTA plasma(n=8) | 96-110 | 103 |
Cell culture media(n=8) | 89-105 | 96 |
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 41.02 | 79.36 | 381.87 | 44.08 | 80.36 | 385.09 |
Standard deviation | 4.88 | 7.56 | 33.64 | 4.82 | 7.88 | 33.43 |
CV (%) | 11.90 | 9.53 | 8.81 | 10.93 | 9.81 | 8.68 |
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