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  • 产品名称：Human ELN (Elastin) CLIA Kit (STJC0000544)

  • 产品型号：STJC0000544
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human ELN (Elastin) CLIA Kit (STJC0000544)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 56.25ng/mL
Detection Limit: 93.75~6000ng/mL
Short Description: This human ELN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: ELN
Gene ID: 2006
Uniprot ID: ELN_HUMAN
Specificity: This kit recognizes Human ELN in samples. No significant cross-reactivity or interference between Human ELN and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
Post Translational Modifications Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine. Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
Function Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
Protein Name Elastin
Tropoelastin
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-1566948
Reactome: R-HSA-2129379
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Extracellular Matrix Of Elastic Fibers
Alternative CLIA Names Elastin CLIA kit
Tropoelastin CLIA kit
ELN CLIA kit
Specificity This kit recognizes Human ELN in samples. No significant cross-reactivity or interference between Human ELN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-104 96
EDTA plasma(n=8) 97-112 102
Cell culture media(n=8) 102-115 108

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 321.00 543.85 2645.01 339.13 561.97 2411.78
Standard deviation 34.44 43.29 204.46 36.56 47.99 252.03
CV (%) 10.73 7.96 7.73 10.78 8.54 10.45
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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