产品详情
简单介绍：
Human CD274 (Programmed cell death 1 ligand 1) Sandwich ELISA (STJE0003050)
详情介绍：
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.09ng/mL |
| Detection Limit: | 0.16~10ng/mL |
| Short Description: | This CD274 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | CD274 |
| Gene ID: | 29126 |
| Uniprot ID: | PD1L1_HUMAN |
| Specificity: | This kit recognizes Human PD-L1 in samples. No significant cross-reactivity or interference between Human PD-L1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Ubiquitinated.STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation. Ubiquitinated by MARCHF8.leading to degradation. |
| Function | Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. |
| Protein Name | Programmed Cell Death 1 Ligand 1 Pd-L1 Pdcd1 Ligand 1 Programmed Death Ligand 1 Hpd-L1 B7 Homolog 1 B7-H1 Cd Antigen Cd274 |
| Database Links | Reactome: R-HSA-389948 Reactome: R-HSA-9701898 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Early Endosome Membrane Recycling Endosome Membrane Nucleus Associates With Cmtm6 At Recycling Endosomes Where It Is Protected From Being Targeted For Lysosomal Degradation Translocates To The Nucleus In Response To Hypoxia Via Its Interaction With Phosphorylated Stat3 Isoform 1: Cell Membrane Isoform 2: Endomembrane System Isoform 4: Secreted |
| Alternative ELISA Names | Programmed Cell Death 1 Ligand 1 ELISA kit Pd-L1 ELISA kit Pdcd1 Ligand 1 ELISA kit Programmed Death Ligand 1 ELISA kit Hpd-L1 ELISA kit B7 Homolog 1 ELISA kit B7-H1 ELISA kit Cd Antigen Cd274 ELISA kit CD274 ELISA kit B7H1 ELISA kit PDCD1L1 ELISA kit PDCD1LG1 ELISA kit PDL1 ELISA kit |
| Specificity | This kit recognizes Human PD-L1 in samples. No significant cross-reactivity or interference between Human PD-L1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 95-111 | 102 |
| EDTA plasma (n=8) | 92-104 | 98 |
| Cell culture media (n=8) | 90-102 | 95 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.54 | 1.35 | 4.76 | 0.49 | 1.29 | 5.23 |
| Standard deviation | 0.03 | 0.08 | 0.17 | 0.03 | 0.05 | 0.18 |
| CV (%) | 5.56 | 5.93 | 3.57 | 6.12 | 3.88 | 3.44 |
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