产品详情
简单介绍：
Human CD276 (Cluster of Differentiation 276) Sandwich ELISA (STJE0019897)
详情介绍：
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.08 ng/mL |
| Detection Limit: | 0.16-10 ng/mL |
| Short Description: | This CD276 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | CD276 |
| Gene ID: | 80381 |
| Uniprot ID: | CD276_HUMAN |
| Specificity: | This kit recognizes Human CD276 in samples. No significant cross-reactivity or interference between Human CD276 and analogues was observed. |
| Sample Type: | serum, plasma and other biological fluids |
| Tissue Specificity | Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta. |
| Function | May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. |
| Protein Name | Cd276 Antigen 4ig-B7-H3 B7 Homolog 3 B7-H3 Costimulatory Molecule Cd Antigen Cd276 |
| Cellular Localisation | Membrane Single-Pass Type I Membrane Protein |
| Alternative ELISA Names | Cd276 Antigen ELISA kit 4ig-B7-H3 ELISA kit B7 Homolog 3 ELISA kit B7-H3 ELISA kit Costimulatory Molecule ELISA kit Cd Antigen Cd276 ELISA kit CD276 ELISA kit B7H3 ELISA kit PSEC0249 ELISA kit UNQ309 ELISA kit PRO352 ELISA kit |
| Specificity | This kit recognizes Human CD276 in samples. No significant cross-reactivity or interference between Human CD276 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 94-105 | 100 |
| EDTA plasma (n=8) | 93-106 | 101 |
| Cell culture media (n=8) | 93-105 | 99 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.48 | 1.04 | 3.68 | 0.46 | 0.98 | 3.53 |
| Standard deviation | 0.03 | 0.06 | 0.20 | 0.03 | 0.06 | 0.14 |
| CV (%) | 6.16 | 5.94 | 5.49 | 5.47 | 5.91 | 3.97 |
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