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  • 产品名称：Human CHGA (Chromogranin-A) Sandwich ELISA (STJE0002444)

  • 产品型号：STJE0002444
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CHGA (Chromogranin-A) Sandwich ELISA (STJE0002444)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This CHGA Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CHGA
Gene ID: 1113
Uniprot ID: CMGA_HUMAN
Specificity: This kit recognizes Human CHGA in samples. No significant cross-reactivity or interference between Human CHGA and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Sulfated on tyrosine residues and/or contains sulfated glycans. O-glycosylated with core 1 or possibly core 8 glycans. Contains chondroitin sulfate (CS).CS attachment is pH-dependent, being observed at mildly acidic conditions of pH 5 but not at neutral pH, and promotes self-assembly in vitro. Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity. Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides.
Function Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas. Catestatin: Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa. Can induce mast cell migration, degranulation and production of cytokines and chemokines. Acts as a potent scavenger of free radicals in vitro. May play a role in the regulation of cardiac function and blood pressure. Serpinin: Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
Protein Name Chromogranin-A
Cga
Pituitary Secretory Protein I
Sp-I Cleaved Into - Vasostatin-1
Vasostatin I - Vasostatin-2
Vasostatin Ii - Ea-92 - Es-43 - Pancreastatin - Ss-18 - Wa-8 - We-14 - Lf-19 - Catestatin
Sl21 - Al-11 - Gv-19 - Gr-44 - Er-37 - Ge-25 - Serpinin-Rrg - Serpinin - P-Glu Serpinin Precursor
Database Links Reactome: R-HSA-6803157
Cellular Localisation Serpinin: Secreted
Cytoplasmic Vesicle
Secretory Vesicle
Pyroglutaminated Serpinin Localizes To Secretory Vesicle
Neuronal Dense Core Vesicle
Secreted
Associated With The Secretory Granule Membrane Through Direct Interaction To Scg3 That In Turn Binds To Cholesterol-Enriched Lipid Rafts In Intragranular Conditions
In Pituitary Gonadotropes
Located In Large Secretory Granules
Alternative ELISA Names Chromogranin-A ELISA kit
Cga ELISA kit
Pituitary Secretory Protein I ELISA kit
Sp-I Cleaved Into - Vasostatin-1 ELISA kit
Vasostatin I - Vasostatin-2 ELISA kit
Vasostatin Ii - Ea-92 - Es-43 - Pancreastatin - Ss-18 - Wa-8 - We-14 - Lf-19 - Catestatin ELISA kit
Sl21 - Al-11 - Gv-19 - Gr-44 - Er-37 - Ge-25 - Serpinin-Rrg - Serpinin - P-Glu Serpinin Precursor ELISA kit
CHGA ELISA kit
Specificity This kit recognizes Human CHGA in samples. No significant cross-reactivity or interference between Human CHGA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-107 99
EDTA plasma (n=8) 89-101 94
Cell culture media (n=8) 87-99 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 183.03 517.46 1372.57 165.11 549.96 1383.06
Standard deviation 12.67 27.58 71.24 11.19 29.15 51.45
CV (%) 6.92 5.33 5.19 6.78 5.30 3.72
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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