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  • 产品名称：Human F12 (Coagulation factor XII) CLIA Kit (STJC0000396)

  • 产品型号：STJC0000396
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human F12 (Coagulation factor XII) CLIA Kit (STJC0000396)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human F12 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: F12
Gene ID: 2161
Uniprot ID: FA12_HUMAN
Specificity: This kit recognizes Human F12 in samples. No significant cross-reactivity or interference between Human F12 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of an NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2. O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.
Function Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
Protein Name Coagulation Factor Xii
Hageman Factor
Haf Cleaved Into - Coagulation Factor Xiia Heavy Chain - Beta-Factor Xiia Part 1 - Coagulation Factor Xiia Light Chain
Beta-Factor Xiia Part 2
Database Links Reactome: R-HSA-140837
Reactome: R-HSA-9657688
Reactome: R-HSA-9657689
Cellular Localisation Secreted
Alternative CLIA Names Coagulation Factor Xii CLIA kit
Hageman Factor CLIA kit
Haf Cleaved Into - Coagulation Factor Xiia Heavy Chain - Beta-Factor Xiia Part 1 - Coagulation Factor Xiia Light Chain CLIA kit
Beta-Factor Xiia Part 2 CLIA kit
F12 CLIA kit
Specificity This kit recognizes Human F12 in samples. No significant cross-reactivity or interference between Human F12 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-104 95
EDTA plasma(n=8) 86-100 93
Cell culture media(n=8) 92-105 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 53.84 103.28 476.02 52.37 104.88 506.95
Standard deviation 5.38 7.28 36.03 4.66 10.80 30.67
CV (%) 9.99 7.05 7.57 8.90 10.30 6.05
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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