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  • 产品名称：Human F13A1 (Coagulation factor XIII A chain) CLIA Kit (STJC0000398)

  • 产品型号：STJC0000398
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human F13A1 (Coagulation factor XIII A chain) CLIA Kit (STJC0000398)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pg/mL
Detection Limit: 12.5~800pg/mL
Short Description: This human F13A1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: F13A1
Gene ID: 2162
Uniprot ID: F13A_HUMAN
Specificity: This kit recognizes Human F13A1 in samples. No significant cross-reactivity or interference between Human F13A1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications The activation peptide is released by thrombin.
Function Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
Protein Name Coagulation Factor Xiii A Chain
Coagulation Factor Xiiia
Protein-Glutamine Gamma-Glutamyltransferase A Chain
Transglutaminase A Chain
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-140875
Reactome: R-HSA-6785807
Cellular Localisation Cytoplasm
Secreted
Secreted Into The Blood Plasma
Cytoplasmic In Most Tissues
But Also Secreted In The Blood Plasma
Alternative CLIA Names Coagulation Factor Xiii A Chain CLIA kit
Coagulation Factor Xiiia CLIA kit
Protein-Glutamine Gamma-Glutamyltransferase A Chain CLIA kit
Transglutaminase A Chain CLIA kit
F13A1 CLIA kit
F13A CLIA kit
Specificity This kit recognizes Human F13A1 in samples. No significant cross-reactivity or interference between Human F13A1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-99 92
EDTA plasma(n=8) 91-103 98
Cell culture media(n=8) 85-97 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 37.66 72.96 305.26 40.70 73.29 277.75
Standard deviation 4.56 7.30 34.40 3.51 6.63 23.64
CV (%) 12.11 10.01 11.27 8.62 9.05 8.51
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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