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  • 产品名称：Human F2 (Prothrombin) Sandwich ELISA (STJE0003231)

  • 产品型号：STJE0003231
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human F2 (Prothrombin) Sandwich ELISA (STJE0003231)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 5.63ng/mL
Detection Limit: 9.38~600ng/mL
Short Description: This F2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: F2
Gene ID: 2147
Uniprot ID: THRB_HUMAN
Specificity: This kit recognizes Human PT in samples. No significant cross-reactivity or interference between Human PT and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin. N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). In the penultimate step of the coagulation cascade, prothrombin is converted to thrombin by the prothrombinase complex composed of factor Xa (F10), cofactor Va (F5), and phospholipids. This activation requires factor Xa-catalyzed sequential cleavage at 2 sites, Arg-314 and Arg-363, along 2 possible pathways. In the first pathway, the first cleavage occurs at Arg-314, leading to the formation of the inactive intermediate prethrombin-2. This pathway preferentially occurs on platelets and in the absence of cofactor Va. In the second pathway, the first cleavage occurs at Arg-363, which separates protease domain into 2 chains that remain connected through a disulfide bond and generates the active intermediate meizothrombin. The presence of cofactor Va directs activation along the meizothrombin pathway and greatly accelerates the rate of cleavage at Arg-363, but has a smaller effect on the cleavage of meizothrombin at Arg-314. Meizothrombin accumulates as an intermediate when prothrombinase is assembled on the membrane of red blood cells.
Function Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
Protein Name Prothrombin
Coagulation Factor Ii Cleaved Into - Activation Peptide Fragment 1 - Activation Peptide Fragment 2 - Thrombin Light Chain - Thrombin Heavy Chain
Database Links Reactome: R-HSA-140837
Reactome: R-HSA-140875
Reactome: R-HSA-159740
Reactome: R-HSA-159763
Reactome: R-HSA-159782
Reactome: R-HSA-202733
Reactome: R-HSA-375276
Reactome: R-HSA-381426
Reactome: R-HSA-416476
Reactome: R-HSA-456926
Reactome: R-HSA-76009
Reactome: R-HSA-9657688
Reactome: R-HSA-9672391
Reactome: R-HSA-977606
Cellular Localisation Secreted
Extracellular Space
Alternative ELISA Names Prothrombin ELISA kit
Coagulation Factor Ii Cleaved Into - Activation Peptide Fragment 1 - Activation Peptide Fragment 2 - Thrombin Light Chain - Thrombin Heavy Chain ELISA kit
F2 ELISA kit
Specificity This kit recognizes Human PT in samples. No significant cross-reactivity or interference between Human PT and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-103 94
EDTA plasma (n=8) 91-106 97
Cell culture media (n=8) 93-106 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 28.24 72.85 262.16 30.55 68.88 263.52
Standard deviation 1.52 4.14 14.39 1.96 3.35 13.70
CV (%) 5.38 5.68 5.49 6.42 4.86 5.20
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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