产品详情
简单介绍:
Human FABP5 (Fatty acid-binding protein 5) Sandwich ELISA (STJE0002708)
详情介绍:
Applications: | ELISA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 0.19ng/mL |
Detection Limit: | 0.31~20ng/mL |
Short Description: | This FABP5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | FABP5 |
Gene ID: | 2171 |
Uniprot ID: | FABP5_HUMAN |
Specificity: | This kit recognizes Human FABP5 in samples. No significant cross-reactivity or interference between Human FABP5 and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Function | Intracellular carrier for long-chain fatty acids and related active lipids, such as endocannabinoids, that regulate the metabolism and actions of the ligands they bind. In addition to the cytosolic transport, selectively delivers specific fatty acids from the cytosol to the nucleus, wherein they activate nuclear receptors. Delivers retinoic acid to the nuclear receptor peroxisome proliferator-activated receptor delta.which promotes proliferation and survival. May also serve as a synaptic carrier of endocannabinoid at central synapses and thus controls retrograde endocannabinoid signaling. Modulates inflammation by regulating PTGES induction via NF-kappa-B activation, and prostaglandin E2 (PGE2) biosynthesis during inflammation. May be involved in keratinocyte differentiation. |
Protein Name | Fatty Acid-Binding Protein 5 Epidermal-Type Fatty Acid-Binding Protein E-Fabp Fatty Acid-Binding Protein - Epidermal Psoriasis-Associated Fatty Acid-Binding Protein Homolog Pa-Fabp |
Database Links | Reactome: R-HSA-163560 Reactome: R-HSA-5362517 Reactome: R-HSA-6798695 |
Cellular Localisation | Cytoplasm Nucleus Synapse Postsynaptic Density Secreted Localizes Primarily To The Cytoplasm Upon Certain Ligand Binding A Conformation Change Exposes A Nuclear Localization Motif And The Protein Is Transported Into Nucleus Secreted By Astrocytes But Not By Neurons |
Alternative ELISA Names | Fatty Acid-Binding Protein 5 ELISA kit Epidermal-Type Fatty Acid-Binding Protein ELISA kit E-Fabp ELISA kit Fatty Acid-Binding Protein - Epidermal ELISA kit Psoriasis-Associated Fatty Acid-Binding Protein Homolog ELISA kit Pa-Fabp ELISA kit FABP5 ELISA kit |
Specificity | This kit recognizes Human FABP5 in samples. No significant cross-reactivity or interference between Human FABP5 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item Specifications Storage
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Sample Type Range (%) Average Recovery (%)
Serum(n=8) | 93-111 | 101 |
EDTA plasma (n=8) | 89-100 | 95 |
Cell culture media (n=8) | 90-103 | 96 |
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 1.03 | 2.47 | 8.12 | 1.02 | 2.43 | 7.93 |
Standard deviation | 0.06 | 0.12 | 0.42 | 0.05 | 0.13 | 0.38 |
CV (%) | 5.83 | 4.86 | 5.17 | 4.90 | 5.35 | 4.79 |
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