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  • 产品名称：Human FBLN1 (Fibulin-1) Sandwich ELISA (STJE0003154)

  • 产品型号：STJE0003154
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human FBLN1 (Fibulin-1) Sandwich ELISA (STJE0003154)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.38ng/mL
Detection Limit: 0.63~40ng/mL
Short Description: This FBLN1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: FBLN1
Gene ID: 2192
Uniprot ID: FBLN1_HUMAN
Specificity: This kit recognizes Human FBLN1 in samples. No significant cross-reactivity or interference between Human FBLN1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP.
Protein Name Fibulin-1
Fibl-1
Database Links Reactome: R-HSA-2129379
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative ELISA Names Fibulin-1 ELISA kit
Fibl-1 ELISA kit
FBLN1 ELISA kit
PP213 ELISA kit
Specificity This kit recognizes Human FBLN1 in samples. No significant cross-reactivity or interference between Human FBLN1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-98 92
EDTA plasma (n=8) 94-110 102
Cell culture media (n=8) 86-99 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.98 4.26 16.72 2.03 4.62 15.87
Standard deviation 0.12 0.23 0.69 0.14 0.26 0.78
CV (%) 6.06 5.40 4.13 6.90 5.63 4.91
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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