产品详情
简单介绍：
Human FGF1 (Fibroblast growth factor 1) CLIA Kit (STJC0000066)
详情介绍：
| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 7.5pg/mL |
| Detection Limit: | 12.5~800pg/mL |
| Short Description: | This human FGF1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | FGF1 |
| Gene ID: | 2246 |
| Uniprot ID: | FGF1_HUMAN |
| Specificity: | This kit recognizes Human AFGF/FGF1 in samples. No significant cross-reactivity or interference between Human AFGF/FGF1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Tissue Specificity | Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle. |
| Post Translational Modifications | In the nucleus, phosphorylated by PKC/PRKCD. |
| Function | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis. |
| Protein Name | Fibroblast Growth Factor 1 Fgf-1 Acidic Fibroblast Growth Factor Afgf Endothelial Cell Growth Factor Ecgf Heparin-Binding Growth Factor 1 Hbgf-1 |
| Database Links | Reactome: R-HSA-109704 Reactome: R-HSA-1257604 Reactome: R-HSA-1839122 Reactome: R-HSA-1839130 Reactome: R-HSA-190322 Reactome: R-HSA-190370 Reactome: R-HSA-190371 Reactome: R-HSA-190372 Reactome: R-HSA-190373 Reactome: R-HSA-190375 Reactome: R-HSA-190377 Reactome: R-HSA-2033519 Reactome: R-HSA-2219530 Reactome: R-HSA-5654219 Reactome: R-HSA-5654221 Reactome: R-HSA-5654227 Reactome: R-HSA-5654228 Reactome: R-HSA-5654687 Reactome: R-HSA-5654688 Reactome: R-HSA-5654689 Reactome: R-HSA-5654693 Reactome: R-HSA-5654695 Reactome: R-HSA-5654699 Reactome: R-HSA-5654700 Reactome: R-HSA-5654704 Reactome: R-HSA-5654706 Reactome: R-HSA-5654710 Reactome: R-HSA-5654712 Reactome: R-HSA-5654719 Reactome: R-HSA-5654720 Reactome: R-HSA-5654726 Reactome: R-HSA-5654727 Reactome: R-HSA-5654732 Reactome: R-HSA-5654733 Reactome: R-HSA-5655253 Reactome: R-HSA-5655302 Reactome: R-HSA-5655332 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-8851708 |
| Cellular Localisation | Secreted Cytoplasm Cell Cortex Cytosol Nucleus Lacks A Cleavable Signal Sequence Within The Cytoplasm It Is Transported To The Cell Membrane And Then Secreted By A Non-Classical Pathway That Requires Cu(2+) Ions And S100a13 Secreted In A Complex With Syt1 Binding Of Exogenous Fgf1 To Fgfr Facilitates Endocytosis Followed By Translocation Of Fgf1 Across Endosomal Membrane Into The Cytosol Nuclear Import From The Cytosol Requires The Classical Nuclear Import Machinery Involving Proteins Kpna1 And Kpnb1 As Well As Lrrc59 |
| Alternative CLIA Names | Fibroblast Growth Factor 1 CLIA kit Fgf-1 CLIA kit Acidic Fibroblast Growth Factor CLIA kit Afgf CLIA kit Endothelial Cell Growth Factor CLIA kit Ecgf CLIA kit Heparin-Binding Growth Factor 1 CLIA kit Hbgf-1 CLIA kit FGF1 CLIA kit FGFA CLIA kit |
| Specificity | This kit recognizes Human AFGF/FGF1 in samples. No significant cross-reactivity or interference between Human AFGF/FGF1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 95-111 | 103 |
| EDTA plasma(n=8) | 94-107 | 101 |
| Cell culture media(n=8) | 88-100 | 93 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 42.84 | 82.30 | 327.26 | 40.45 | 89.02 | 353.66 |
| Standard deviation | 4.36 | 6.32 | 28.01 | 4.91 | 8.46 | 37.84 |
| CV (%) | 10.18 | 7.68 | 8.56 | 12.14 | 9.50 | 10.70 |
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