产品详情
简单介绍:
Human FGF21 (Fibroblast growth factor 21) CLIA Kit (STJC0000068)
详情介绍:
Applications: | CLIA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 7.5pg/mL |
Detection Limit: | 12.5~800pg/mL |
Short Description: | This human FGF21 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Chemiluminescence |
Gene Symbol: | FGF21 |
Gene ID: | 26291 |
Uniprot ID: | FGF21_HUMAN |
Specificity: | This kit recognizes Human FGF21 in samples. No significant cross-reactivity or interference between Human FGF21 and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Function | Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity. |
Protein Name | Fibroblast Growth Factor 21 Fgf-21 |
Database Links | Reactome: R-HSA-189200 Reactome: R-HSA-8963889 |
Cellular Localisation | Secreted |
Alternative CLIA Names | Fibroblast Growth Factor 21 CLIA kit Fgf-21 CLIA kit FGF21 CLIA kit UNQ3115 CLIA kit PRO10196 CLIA kit |
Specificity | This kit recognizes Human FGF21 in samples. No significant cross-reactivity or interference between Human FGF21 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Serum(n=8) | 85-99 | 92 |
EDTA plasma(n=8) | 99-113 | 106 |
Cell culture media(n=8) | 97-113 | 104 |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 39.32 | 81.85 | 297.58 | 39.70 | 80.40 | 316.22 |
Standard deviation | 4.68 | 7.07 | 23.48 | 3.50 | 7.26 | 28.62 |
CV (%) | 11.90 | 8.64 | 7.89 | 8.82 | 9.03 | 9.05 |
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