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  • 产品名称：Human FLNB (Filamin-B) CLIA Kit (STJC0000882)

  • 产品型号：STJC0000882
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human FLNB (Filamin-B) CLIA Kit (STJC0000882)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This human FLNB kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: FLNB
Gene ID: 2317
Uniprot ID: FLNB_HUMAN
Specificity: This kit recognizes Human FLN Beta in samples. No significant cross-reactivity or interference between Human FLN Beta and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Ubiquitous. Isoform 1 and isoform 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells (HUVEC), retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord, platelet and Daudi cells. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer disease (AD). Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart.
Post Translational Modifications ISGylation prevents ability to interact with the upstream activators of the JNK cascade and inhibits IFNA-induced JNK signaling. Ubiquitination by a SCF-like complex containing ASB2 isoform 1 leads to proteasomal degradation which promotes muscle differentiation.
Function Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro.
Protein Name Filamin-B
Fln-B
Abp-278
Abp-280 Homolog
Actin-Binding-Like Protein
Beta-Filamin
Filamin Homolog 1
Fh1
Filamin-3
Thyroid Autoantigen
Truncated Actin-Binding Protein
Truncated Abp
Database Links Reactome: R-HSA-1169408
Cellular Localisation Isoform 1: Cytoplasm
Cell Cortex
Cytoplasm
Cytoskeleton
Stress Fiber
Myofibril
Sarcomere
Z Line
In Differentiating Myotubes
Isoform 1
Isoform 2 And Isoform 3 Are Localized Diffusely Throughout The Cytoplasm With Regions Of Enrichment At The Longitudinal Actin Stress Fiber
In Differentiated Tubes
Isoform 1 Is Also Detected Within The Z-Lines
Isoform 2: Cytoplasm
Isoform 3: Cytoplasm
Isoform 6: Cytoplasm
Polarized At The Periphery Of Myotubes
Alternative CLIA Names Filamin-B CLIA kit
Fln-B CLIA kit
Abp-278 CLIA kit
Abp-280 Homolog CLIA kit
Actin-Binding-Like Protein CLIA kit
Beta-Filamin CLIA kit
Filamin Homolog 1 CLIA kit
Fh1 CLIA kit
Filamin-3 CLIA kit
Thyroid Autoantigen CLIA kit
Truncated Actin-Binding Protein CLIA kit
Truncated Abp CLIA kit
FLNB CLIA kit
FLN1L CLIA kit
FLN3 CLIA kit
TABP CLIA kit
TAP CLIA kit
Specificity This kit recognizes Human FLNβ in samples. No significant cross-reactivity or interference between Human FLNβ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-104 99
EDTA plasma(n=8) 86-100 92
Cell culture media(n=8) 99-113 106

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 219.46 549.44 1535.63 221.24 523.93 1556.80
Standard deviation 18.17 60.49 175.06 21.55 39.40 153.97
CV (%) 8.28 11.01 11.40 9.74 7.52 9.89
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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