产品详情
简单介绍：
Human FN1 (Fibronectin) Sandwich ELISA (STJE0002036)
详情介绍：
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.94ng/mL |
| Detection Limit: | 1.56~100ng/mL |
| Short Description: | This FN1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | FN1 |
| Gene ID: | 2335 |
| Uniprot ID: | FINC_HUMAN |
| Specificity: | This kit recognizes Human FN in samples. No significant cross-reactivity or interference between Human FN and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Sulfated. It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylated by FAM20C in the extracellular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation. Serotonylated on Gln residues by TGM2 in response to hypoxia. |
| Function | Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation. Anastellin: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling. |
| Protein Name | Fibronectin Fn Cold-Insoluble Globulin Cig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3 |
| Database Links | Reactome: R-HSA-114608 Reactome: R-HSA-1474228 Reactome: R-HSA-1474244 Reactome: R-HSA-1566977 Reactome: R-HSA-202733 Reactome: R-HSA-2129379 Reactome: R-HSA-216083 Reactome: R-HSA-3000170 Reactome: R-HSA-3000171 Reactome: R-HSA-3000178 Reactome: R-HSA-354192 Reactome: R-HSA-354194 Reactome: R-HSA-372708 Reactome: R-HSA-381426 Reactome: R-HSA-5674135 Reactome: R-HSA-6785807 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-8874081 Reactome: R-HSA-8957275 Reactome: R-HSA-9634597 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9700645 Reactome: R-HSA-9725370 Reactome: R-HSA-9725371 |
| Cellular Localisation | Secreted Extracellular Space Extracellular Matrix |
| Alternative ELISA Names | Fibronectin ELISA kit Fn ELISA kit Cold-Insoluble Globulin ELISA kit Cig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3 ELISA kit FN1 ELISA kit FN ELISA kit |
| Specificity | This kit recognizes Human FN in samples. No significant cross-reactivity or interference between Human FN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 89-102 | 96 |
| EDTA plasma (n=8) | 94-110 | 101 |
| Cell culture media (n=8) | 91-105 | 98 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 5.02 | 14.93 | 40.64 | 4.58 | 15.48 | 37.14 |
| Standard deviation | 0.29 | 0.67 | 1.38 | 0.24 | 0.64 | 1.15 |
| CV (%) | 5.78 | 4.49 | 3.40 | 5.24 | 4.13 | 3.10 |
相关信息
![Anti-14-3-3 beta/Theta antibody (1-100) [S1MR] (STJ11103651)](http://y1.yzimgs.com/uploads/465546/2024520-20551366.png?imageView2/2/w/200/h/200)
- 细胞培养玄学-MRC-5细胞 2025-01-07
- Ocean NanoTech定制的详细步骤 2021-10-31
- Abbiotec代理的产品得到重复验证2020 2021-10-31
- click chemistry tools数据结果比... 2021-10-31
- LaysanBio代理商相关文献资料2020 2021-10-31
- zyagen Laboratories代理的产品得到... 2021-06-20
- Toxin Technologies代理商相关文献资... 2021-03-14
- 热烈祝贺玉博生物正式代理biocolor公司试剂产品... 2021-03-14
- 热烈祝贺玉博生物正式代理cytognos公司试剂产品... 2021-03-14
- Gelest代理的产品得到重复验证2020 2021-03-14
