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  • 产品名称：Human GAD2 (Glutamate decarboxylase 2) Sandwich ELISA (STJE0004081)

  • 产品型号：STJE0004081
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human GAD2 (Glutamate decarboxylase 2) Sandwich ELISA (STJE0004081)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 46.88pg/mL
Detection Limit: 78.13~5000pg/mL
Short Description: This GAD2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: GAD2
Gene ID: 2572
Uniprot ID: DCE2_HUMAN
Specificity: This kit recognizes Human GAD2 in samples. No significant cross-reactivity or interference between Human GAD2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Catalyzes the production of GABA.
Protein Name Glutamate Decarboxylase 2
65 Kda Glutamic Acid Decarboxylase
Gad-65
Glutamate Decarboxylase 65 Kda Isoform
Database Links Reactome: R-HSA-888568
Reactome: R-HSA-888590
Reactome: R-HSA-9022927
Cellular Localisation Cytoplasm
Cytosol
Cytoplasmic Vesicle
Presynaptic Cell Membrane
Lipid-Anchor
Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Associated To Cytoplasmic Vesicles
In Neurons
Cytosolic Leaflet Of Golgi Membranes And Presynaptic Clusters
Alternative ELISA Names Glutamate Decarboxylase 2 ELISA kit
65 Kda Glutamic Acid Decarboxylase ELISA kit
Gad-65 ELISA kit
Glutamate Decarboxylase 65 Kda Isoform ELISA kit
GAD2 ELISA kit
GAD65 ELISA kit
Specificity This kit recognizes Human GAD2 in samples. No significant cross-reactivity or interference between Human GAD2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-103 97
EDTA plasma (n=8) 101-107 104
Cell culture media (n=8) 87-95 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 209.71 556.55 2120.82 232.74 603.91 2042.33
Standard deviation 11.32 26.10 104.13 12.75 28.20 88.02
CV (%) 5.40 4.69 4.91 5.48 4.67 4.31
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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