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  • 产品名称：Human GAP43 (Neuromodulin) CLIA Kit (STJC0000838)

  • 产品型号：STJC0000838
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human GAP43 (Neuromodulin) CLIA Kit (STJC0000838)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human GAP43 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: GAP43
Gene ID: 2596
Uniprot ID: NEUM_HUMAN
Specificity: This kit recognizes Human GAP43 in samples. No significant cross-reactivity or interference between Human GAP43 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylated. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Palmitoylated by ZDHHC3. Palmitoylation is regulated by ARF6 and is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2.
Function This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
Protein Name Neuromodulin
Axonal Membrane Protein Gap-43
Growth-Associated Protein 43
Neural Phosphoprotein B-50
Pp46
Database Links Reactome: R-HSA-373760
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Projection
Growth Cone Membrane
Synapse
Filopodium Membrane
Perikaryon
Dendrite
Axon
Cytoplasm
Cytoplasmic Surface Of Growth Cone And Synaptic Plasma Membranes
Alternative CLIA Names Neuromodulin CLIA kit
Axonal Membrane Protein Gap-43 CLIA kit
Growth-Associated Protein 43 CLIA kit
Neural Phosphoprotein B-50 CLIA kit
Pp46 CLIA kit
GAP43 CLIA kit
Specificity This kit recognizes Human GAP43 in samples. No significant cross-reactivity or interference between Human GAP43 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-112 106
EDTA plasma(n=8) 89-101 96
Cell culture media(n=8) 93-107 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 94.41 287.04 773.36 102.98 274.69 735.86
Standard deviation 7.59 25.03 49.96 10.74 32.47 61.52
CV (%) 8.04 8.72 6.46 10.43 11.82 8.36
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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