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  • 产品名称：Human GDF9 (Growth/differentiation factor 9) Sandwich ELISA (STJE0003319)

  • 产品型号：STJE0003319
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human GDF9 (Growth/differentiation factor 9) Sandwich ELISA (STJE0003319)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This GDF9 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: GDF9
Gene ID: 2661
Uniprot ID: GDF9_HUMAN
Specificity: This kit recognizes Human GDF9 in samples. No significant cross-reactivity or interference between Human GDF9 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylated.phosphorylation is critical for GDF9 function. In vitro, can be phosphorylated by CK at Ser-325.
Function Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.
Protein Name Growth/Differentiation Factor 9
Gdf-9
Cellular Localisation Secreted
Alternative ELISA Names Growth/Differentiation Factor 9 ELISA kit
Gdf-9 ELISA kit
GDF9 ELISA kit
Specificity This kit recognizes Human GDF9 in samples. No significant cross-reactivity or interference between Human GDF9 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-102 95
EDTA plasma (n=8) 85-100 92
Cell culture media (n=8) 86-96 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.50 0.84 4.97 0.51 0.82 4.85
Standard deviation 0.03 0.04 0.23 0.03 0.04 0.15
CV (%) 6.00 4.76 4.63 5.88 4.88 3.09
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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